Svobodová M
Department of Parasitology, Faculty of Science, Charles University, Vinicná 7, 128 44 2, Prague, Czech Republic.
Acta Trop. 2000 Oct 2;76(3):285-8. doi: 10.1016/s0001-706x(00)00105-4.
Gut lectins of the insect vectors play important roles in the transmission of pathogens. Galactosamine, a sandfly midgut lectin inhibitor, was previously demonstrated to increase the intensity of Leishmania infection in sandflies; it was suggested that this was due to the inhibition of sandfly midgut lectin. However, galactosamine might also enhance Leishmania growth in the sandfly. Therefore, the aim of this work was to study the effect of galactosamine and other carbohydrates on Leishmania in vitro. At 50 mM concentration, galactosamine inhibits growth of promastigotes, at 10 mM it induces morphological changes similar to that seen in infected sandflies. Glucosamine effect is less pronounced, galactose has no effect. This suggests that the effect of galactosamine on Leishmania differs in vivo and in vitro. Thus, galactosamine does not enhance Leishmania development in sandflies directly, but rather interferes with some aspect of sandfly physiology (e.g. proteinase and/or lectin activity).
昆虫媒介的肠道凝集素在病原体传播中发挥着重要作用。半乳糖胺是一种白蛉中肠凝集素抑制剂，此前已证明它会增加白蛉体内利什曼原虫的感染强度；据推测，这是由于对白蛉中肠凝集素的抑制作用。然而，半乳糖胺也可能促进利什曼原虫在白蛉体内的生长。因此，本研究的目的是在体外研究半乳糖胺和其他碳水化合物对利什曼原虫的影响。在50 mM浓度下，半乳糖胺抑制前鞭毛体的生长，在10 mM时，它会诱导出与感染白蛉中所见相似的形态变化。氨基葡萄糖的作用不太明显，半乳糖则没有作用。这表明半乳糖胺对利什曼原虫的影响在体内和体外有所不同。因此，半乳糖胺不会直接促进利什曼原虫在白蛉体内的发育，而是干扰白蛉生理的某些方面（如蛋白酶和/或凝集素活性）。
