Cassity T R, Kolodziej B J, Pfister R M
Microbios. 1978;21(85-86):153-60.
The ultrastructure of the firmly adherent capsule produced by Bacillus megaterium cultured on fructose mineral salts medium was examined using thin sectioning, freeze-etching, and critical point drying by transmission and scanning electron microscopy. The capsule material was shown to be fibrillar, with most fibrils containing bulbous protrusions. Two types of fibres were resolved. These were termed primary and cross-linking fibres. Primary fibres originated at the cell wall and had a diameter of 34-50 nm. They also contained bulbous protrusions and enlarged areas where branching occurred. Cross-linking fibres connected the primary fibres. The cross-linking fibres were much smaller, usually 15 micro m in diameter, and contained few enlarged areas. The primary fibres originated at sites on the cell wall approximately equidistant and 0.26 micro m apart.
使用超薄切片、冷冻蚀刻以及临界点干燥技术，通过透射电子显微镜和扫描电子显微镜对巨大芽孢杆菌在果糖矿物盐培养基上培养产生的牢固附着的荚膜的超微结构进行了检查。结果显示，荚膜物质呈纤维状，大多数纤维含有球状突起。分辨出了两种类型的纤维。它们被称为初级纤维和交联纤维。初级纤维起源于细胞壁，直径为34 - 50纳米。它们还含有球状突起和发生分支的扩大区域。交联纤维连接初级纤维。交联纤维要小得多，通常直径为15微米，且几乎没有扩大区域。初级纤维起源于细胞壁上距离大致相等且相距0.26微米的位点。
