Beaman T C, Pankratz H S, Gerhardt P
J Bacteriol. 1972 Mar;109(3):1198-209. doi: 10.1128/jb.109.3.1198-1209.1972.
Spores of selected strains of Bacillus megaterium were prepared by various methods and examined with the electron microscope. An exosporium like that of B. cereus, with a nap and basal layer, was found in spores of a B. megaterium strain that reportedly contains a capsule-like exosporium. The exosporium occasionally appeared to be doubled or have an apical opening. Pili-like filaments were discerned on the surface. Beneath the exosporium were found large deposits of planar inclusions, which in cross section appeared laminated and in surface views consisted of a patchwork of striated packets with a periodicity of approximately 5 nm. The inclusions were usually attached to the exosporium, but in ultrastructure they differed from both the exosporium and coat. In two other strains of B. megaterium, one or two coats occurred but a typical exosporium was not present.
通过多种方法制备了巨大芽孢杆菌选定菌株的孢子，并使用电子显微镜进行了检查。在一株据报道含有类似荚膜外孢子囊的巨大芽孢杆菌菌株的孢子中，发现了一种类似于蜡样芽孢杆菌的外孢子囊，具有绒毛层和基底层。外孢子囊偶尔似乎是双层的或有顶端开口。在其表面发现了类似菌毛的细丝。在外孢子囊下方发现了大量平面内含物沉积物，其横截面呈层状，在表面观察中由周期性约为5纳米的条纹状小包拼凑而成。这些内含物通常附着在外孢子囊上，但在超微结构上它们与外孢子囊和芽孢衣都不同。在另外两株巨大芽孢杆菌中，出现了一层或两层芽孢衣，但没有典型的外孢子囊。
