原发性胃癌中21q22等位基因缺失新靶区域的定位

Mapping of a new target region of allelic loss at 21q22 in primary gastric cancers.

作者信息

Park W S, Oh R R, Park J Y, Yoo N J, Lee S H, Shin M S, Kim S Y, Kim Y S, Lee J H, Kim H S, An W G, Lee J Y

机构信息

Department of Pathology and Genetic Oncology Laboratory, College of Medicine, The Catholic University of Korea, 137-701, Seoul, South Korea.

出版信息

Cancer Lett. 2000 Oct 16;159(1):15-21. doi: 10.1016/s0304-3835(00)00525-5.

DOI:10.1016/s0304-3835(00)00525-5

PMID:10974401

Abstract

To determine the minimal region of deletion on 21q22 in gastric cancer, we performed a high-density loss of heterozygosity (LOH) study with eight polymorphic microsatellite markers. Among the 43 tumors examined, 20 (50%) of 40 informative carcinomas showed LOH at one or more loci. The peak LOH frequency was identified at D21S1820 (34.2%) in 21q22.3. This data suggests that this locus might harbor a new tumor suppressor gene in an area <0.332 Mb in physical map distance defined by D21S1820 and D21S49. Thus, we speculate that trefoil factor family 1 (TFF1), located in this narrow region, might be the most probable candidate gene involved in gastric cancer carcinogenesis.

摘要

为了确定胃癌中21q22上的最小缺失区域，我们使用八个多态性微卫星标记进行了高密度杂合性缺失（LOH）研究。在检测的43个肿瘤中，40个信息丰富的癌中有20个（50%）在一个或多个位点显示出LOH。在21q22.3的D21S1820处确定了最高的LOH频率（34.2%）。该数据表明，在由D21S1820和D21S49定义的物理图谱距离<0.332 Mb的区域中，该位点可能含有一个新的肿瘤抑制基因。因此，我们推测位于这个狭窄区域的三叶因子家族1（TFF1）可能是参与胃癌致癌作用的最有可能的候选基因。