Igarashi J, Nimura Y, Fujimori M, Mihara M, Adachi W, Kageyama H, Nakagawara A
Second Department of Surgery, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan.
Jpn J Cancer Res. 2000 Aug;91(8):797-801. doi: 10.1111/j.1349-7006.2000.tb01016.x.
In order to identify the region on distal chromosome 1p that is thought to include one or more tumor suppressor genes for gastric carcinoma, 39 gastric carcinomas were examined for allelic loss using 11 polymorphic microsatellite markers and 1 marker of single strand conformation polymorphism. Loss of heterozygosity (LOH) was found in 18 (46%) of 39 informative patients. The regions with high frequency of loss of heterozygosity were the loci at D1S548 (6 / 17; 35.3%) and D1S2843 (7 / 20; 35%), and we found three commonly deleted regions on chromosome 1p35-pter. The frequency of allelic loss in the region of chromosome 1p35-pter was significantly associated with advanced-stage gastric carcinoma, but not with early-stage tumor or with the histology. These results suggest that allelic loss at chromosome 1p35-pter may play a role in the progression of gastric carcinoma.
为了确定被认为包含一个或多个胃癌肿瘤抑制基因的1号染色体短臂远端区域,使用11个多态性微卫星标记和1个单链构象多态性标记对39例胃癌进行了等位基因缺失检测。在39例信息充分的患者中,有18例(46%)发现杂合性缺失(LOH)。杂合性缺失高频区域为D1S548位点(6 / 17;35.3%)和D1S2843位点(7 / 20;35%),并且我们在1号染色体短臂35区至末端发现了三个常见的缺失区域。1号染色体短臂35区至末端区域的等位基因缺失频率与进展期胃癌显著相关,但与早期肿瘤或组织学无关。这些结果表明,1号染色体短臂35区至末端的等位基因缺失可能在胃癌进展中起作用。