Ishigami S, Natsugoe S, Tokuda K, Nakajo A, Xiangming C, Iwashige H, Aridome K, Hokita S, Aikou T
First Department of Surgery, Kagoshima University School of Medicine, 8-35-1 Sakuragaoka, 890-8520, Kagoshima, Japan.
Cancer Lett. 2000 Oct 16;159(1):103-8. doi: 10.1016/s0304-3835(00)00542-5.
Intratumoral natural killer cells (NKC) and dendritic cells (DC) may affect the clinical features of various gastrointestinal cancers. However, the relationship between intratumoral NKC and DC remains unclear. We examined 169 patients with gastric cancer who underwent gastrectomy at Kagoshima University Hospital. Immunohistochemical staining of CD57 and S-100-protein was performed to evaluate NKC and DC infiltration, respectively. A total of 25 areas containing pericancerous tissue were selected for determining the number of NKC and DC under high power microscopy (x400). Patients were classified into two groups according to NKC and DC population. Intratumoral lymphocytic infiltration was also calculated in 15 areas with a high power (x400) objective. The degree of NKC and DC infiltration was gradually decreased according to the progression of nodal involvement. Patients with many NKC infiltration had a lower positivity of lymph node metastasis and lymphatic invasion than patients with little NKC infiltration. DC infiltration was also negatively correlated with depth of invasion, lymph node metastasis and curativity. DC infiltration was positively correlated with lymphocytic infiltration (P=0.01. r=0.6). The 5-year survival rates of patients with many NKC infiltration and patients with DC many infiltration were 75 and 78%, respectively, both of which were significantly better than that of patients with little NKC and DC infiltration (P<0.05). NKC may be activated without DC or intratumoral lymphocytes. Intratumoral NKC may act as an independent immunologic effector against tumor cells, unlike DC.
肿瘤内自然杀伤细胞（NKC）和树突状细胞（DC）可能影响各种胃肠道癌症的临床特征。然而，肿瘤内NKC与DC之间的关系仍不清楚。我们研究了169例在鹿儿岛大学医院接受胃切除术的胃癌患者。分别进行CD57和S-100蛋白的免疫组织化学染色以评估NKC和DC浸润。总共选择25个包含癌旁组织的区域，在高倍显微镜（×400）下确定NKC和DC的数量。根据NKC和DC的数量将患者分为两组。还在15个高倍（×400）物镜区域计算肿瘤内淋巴细胞浸润情况。根据淋巴结受累的进展，NKC和DC浸润程度逐渐降低。NKC浸润多的患者与NKC浸润少的患者相比，淋巴结转移和淋巴管侵犯的阳性率较低。DC浸润也与浸润深度、淋巴结转移和治愈率呈负相关。DC浸润与淋巴细胞浸润呈正相关（P = 0.01，r = 0.6）。NKC浸润多的患者和DC浸润多的患者的5年生存率分别为75%和78%，均显著高于NKC和DC浸润少的患者（P < 0.05）。NKC可能在没有DC或肿瘤内淋巴细胞的情况下被激活。与DC不同，肿瘤内NKC可能作为针对肿瘤细胞的独立免疫效应细胞发挥作用。
