Tsujitani S, Oka A, Kondo A, Ikeguchi M, Maeta M, Kaibara N
Department of Surgery I, Faculty of Medicine, Tottori University, Yonago, Japan.
Cancer. 1995 Mar 15;75(6 Suppl):1478-83. doi: 10.1002/1097-0142(19950315)75:6+<1478::aid-cncr2820751516>3.0.co;2-o.
Dendritic cells (DC), including epidermal Langerhans cells, are potent antigen-presenting cells that can carry and present tumor antigens to helper T-cells. An immunohistochemical study was performed to clarify the relationship between the extent of infiltration by DCs in primary gastric cancer and that in the regional lymph nodes.
Paraffin blocks were prepared for staining with antibody against S-100 protein in 121 cases of gastric cancer. Infiltration of S-100 protein-positive DCs was evaluated in the primary tumor and in the peritumoral, primary, secondary, and tertiary nodes.
The extent of infiltration by DCs into the regional lymph nodes decreased significantly with an increasing distance of the nodes from the primary tumor. Correlation of the extent of such infiltration was observed among the peritumoral, primary, secondary, and tertiary nodes. However, the extent of infiltration into the primary tumor did not correlate with that into the primary, secondary, and tertiary nodes. Infiltration by DCs of the peritumoral nodes correlated with that of the tumor, particularly when lymph node metastasis was absent. There was no significant difference in the extent of infiltration into the peritumoral nodes even when lymph nodes were involved. However, infiltration of DCs increased in the primary and secondary nodes when the primary nodes were involved.
Infiltration of DCs may be regulated in the primary lesion of gastric cancer. The peritumoral lymph nodes serve as transmitters of DCs from the primary tumor to the regional lymph nodes. DCs in the regional lymph nodes are important for establishing immunologic defense mechanisms in cases of gastric cancer when metastasis is absent or limited to the primary nodes.
包括表皮朗格汉斯细胞在内的树突状细胞（DC）是强大的抗原呈递细胞，能够携带并将肿瘤抗原呈递给辅助性T细胞。进行了一项免疫组织化学研究，以阐明原发性胃癌中DC浸润程度与区域淋巴结中DC浸润程度之间的关系。
制备石蜡块，用于对121例胃癌患者进行抗S-100蛋白抗体染色。评估原发性肿瘤以及瘤周、一级、二级和三级淋巴结中S-100蛋白阳性DC的浸润情况。
随着淋巴结与原发性肿瘤距离的增加，DC向区域淋巴结的浸润程度显著降低。在瘤周、一级、二级和三级淋巴结之间观察到这种浸润程度的相关性。然而，原发性肿瘤中的浸润程度与一级、二级和三级淋巴结中的浸润程度无关。瘤周淋巴结中DC的浸润与肿瘤中的浸润相关，尤其是在无淋巴结转移时。即使淋巴结受累，瘤周淋巴结的浸润程度也没有显著差异。然而，当一级淋巴结受累时，一级和二级淋巴结中DC的浸润增加。
胃癌原发性病变中DC的浸润可能受到调控。瘤周淋巴结充当DC从原发性肿瘤向区域淋巴结的传递者。在无转移或转移仅限于一级淋巴结的胃癌病例中，区域淋巴结中的DC对于建立免疫防御机制很重要。
