Beitel G J, Lambie E J, Horvitz H R
Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Gene. 2000 Aug 22;254(1-2):253-63. doi: 10.1016/s0378-1119(00)00296-1.
The Caenorhabditis elegans gene lin-9 functions in an Rb-related pathway that acts antagonistically to a receptor tyrosine kinase/Ras signal transduction pathway controlling vulval induction. We show that lin-9 is also required for the development of the sheath cells in the hermaphrodite gonad and for the development of the male spicule, rays and gonad. lin-9 is transcribed as two alternatively spliced 2.4kb messages, which use two distinct polyadenylation sites and are SL1 trans-spliced. The conceptual translation of lin-9 cDNA sequences predicts proteins of 642 and 644 amino acids with a significant similarity to predicted Drosophila and vertebrate proteins. We suggest that lin-9 is the founding member of a new protein family that functions in Rb-related pathways in many species.
秀丽隐杆线虫基因lin-9在一条与Rb相关的信号通路中发挥作用，该信号通路与控制外阴诱导的受体酪氨酸激酶/Ras信号转导通路起拮抗作用。我们发现，lin-9对于雌雄同体性腺中鞘细胞的发育以及雄性交合刺、射线和性腺的发育也是必需的。lin-9转录为两条选择性剪接的2.4kb信息，它们使用两个不同的聚腺苷酸化位点，并进行SL1反式剪接。lin-9 cDNA序列的概念性翻译预测出642和644个氨基酸的蛋白质，与预测的果蝇和脊椎动物蛋白质具有显著相似性。我们认为lin-9是一个新蛋白质家族的创始成员，该家族在许多物种的Rb相关信号通路中发挥作用。
