Mulieri P J, Okada A, Sassoon D A, McConnell S K, Krauss R S
Department of Biochemistry and Molecular Biology, Mount Sinai School of Medicine, New York, New York 10029, USA.
Dev Dyn. 2000 Sep;219(1):40-9. doi: 10.1002/1097-0177(2000)9999:9999<::AID-DVDY1032>3.0.CO;2-M.
CDO is a cell-surface protein of the immunoglobulin/fibronectin type III repeat family that positively regulates myogenic differentiation in vitro. To gain a better understanding of the role of cdo during vertebrate development, we carried out an extensive in situ hybridization study to characterize its expression pattern from postimplantation to late stages of mouse embryogenesis and in rat brain from E13 to adult. Our results show a broad pattern of cdo expression that is spatially and temporally restricted during embryogenesis. In the central nervous system (CNS), cdo expression is detected as early as E7.5 and maintained in the dorsal ventricular zones of the brain and spinal cord, becoming increasingly restricted in the adult. High levels of cdo are detected in developing sensory organs, such as the eye and ear. Outside the CNS, cdo is expressed mainly in neural crest and mesodermal derivatives, including skeletal muscle precursors. Overall, the highest levels of cdo expression are seen from E9.0 to E15.5. The temporal onset and restricted expression of cdo suggest that cdo plays a role in the determination and/or differentiation of a number of cell types during embryogenesis.
CDO是免疫球蛋白/纤连蛋白III型重复序列家族的一种细胞表面蛋白，在体外对肌源性分化起正向调节作用。为了更好地了解cdo在脊椎动物发育过程中的作用，我们进行了广泛的原位杂交研究，以表征其在小鼠胚胎发育从植入后到晚期以及大鼠从胚胎第13天到成年期大脑中的表达模式。我们的结果显示了cdo广泛的表达模式，在胚胎发育过程中在空间和时间上受到限制。在中枢神经系统（CNS）中，早在胚胎第7.5天就检测到cdo的表达，并在大脑和脊髓的背侧脑室区维持表达，在成年期表达范围越来越局限。在发育中的感觉器官，如眼睛和耳朵中检测到高水平的cdo。在中枢神经系统之外，cdo主要在神经嵴和中胚层衍生物中表达，包括骨骼肌前体。总体而言，在胚胎第9.0天到第15.5天观察到cdo表达的最高水平。cdo在时间上的起始表达和局限表达表明，cdo在胚胎发育过程中对多种细胞类型的决定和/或分化起作用。
