Alvarez R, Cour I, Kanaan A, Benedicto M, Martín-Estefanía C, Arroyo R, Varela de Seijas E, Picazo J J
Servicio de Microbiología, Hospital Universitario San Carlos, Madrid.
Enferm Infecc Microbiol Clin. 2000 May;18(5):223-8.
The multiple sclerosis seems to be the junction between genetics alteration and an unknown environmental factor, that they would originate an autoimmune alteration, that they would be the reason of the inflammation and demyelinization responsible of the disease. Our objective has been the determination of this possible environmental factor and to reach it, we have studied the appearance of Herpesviridae family viruses.
204 blood samples were studied: 102 from relapsing-remitting multiple sclerosis patients (43 were undergoing beta-interferon treatment), and 102 from blood donors with the same age and sex than multiple sclerosis patients. From this samples, we extracted the DNA of peripheral blood mononuclear cells (PBMCs), and we analyzed by polymerase chain reaction (PCR) to detect the appearance of herpes simplex virus, varicella zoster virus, Epstein-Barr virus, cytomegalovirus, human herpesvirus 6 (HHV-6), human herpesvirus 7 and human herpesvirus 8.
a) we only found significative difference (p = 0.0001) in HHV-6: 21.5% donors positive samples (22/102), opposite to 49.02% of positivity in mulytiple sclerosis patients (50/102); b) we didn't found significative differences in none of other viruses studied, between patients treated with beta-interferon and non-treated ones.
Our results suggest us that HHV-6 can play an important role in the multiple sclerosis development. The beta-interferon treatment doesn't affect to DNA prevalence of none of studied viruses.
多发性硬化似乎是基因改变与未知环境因素的交汇点，这些因素会引发自身免疫改变，而自身免疫改变是导致该疾病炎症和脱髓鞘的原因。我们的目标是确定这种可能的环境因素，为此我们研究了疱疹病毒科病毒的出现情况。
研究了204份血样：102份来自复发缓解型多发性硬化患者（43例正在接受β-干扰素治疗），以及102份来自与多发性硬化患者年龄和性别相同的献血者。从这些样本中提取外周血单个核细胞（PBMCs）的DNA，并通过聚合酶链反应（PCR）进行分析，以检测单纯疱疹病毒、水痘带状疱疹病毒、爱泼斯坦-巴尔病毒、巨细胞病毒、人类疱疹病毒6型（HHV-6）、人类疱疹病毒7型和人类疱疹病毒8型的出现情况。
a）我们仅在HHV-6中发现显著差异（p = 0.0001）：献血者阳性样本为21.5%（22/102），而多发性硬化患者的阳性率为49.02%（50/102）；b）在接受β-干扰素治疗的患者和未接受治疗的患者之间，在所研究的其他病毒中均未发现显著差异。
我们的结果表明，HHV-6可能在多发性硬化的发展中起重要作用。β-干扰素治疗不影响所研究病毒的DNA流行率。
