Alvarez-Lafuente R, Fernández-Gutiérrez B, de Miguel S, Jover J A, Rollin R, Loza E, Clemente D, Lamas J R
Service of Rheumatology, Hospital Clínico San Carlos, Profesor Martín, Lagos s/n, 28040 Madrid, Spain.
Ann Rheum Dis. 2005 Sep;64(9):1357-9. doi: 10.1136/ard.2004.033514.
To determine whether the human herpes viruses, cytomegalovirus (CMV), Epstein-Barr virus (EBV), and human herpesvirus 6 (HHV-6), are detectable in serum and peripheral blood mononuclear cells (PBMCs) of patients with rheumatoid arthritis (RA).
133 PBMC samples (61 RA, 72 healthy donors) and 136 serum samples (59 RA, 77 healthy donors) were analysed by quantitative real time polymerase chain reaction for DNA prevalence and viral load of HHV-6, EBV, and CMV.
For PBMC samples significant differences were found for EBV in DNA prevalence (56% in RA v 33% in controls, p = 0.009) and viral load (copies/microg DNA 0-592.3 for RA v 0-40.4 for controls, p = 0.001). For serum samples a significant difference was found for HHV-6 DNA prevalence (10% in RA v 0% in controls, p = 0.006) and viral load (copies/microg DNA 0-529.1 for RA v 0 for controls, p = 0.007).
Herpes viruses may have a role in RA, although alternative explanations are possible: (a) defects in cellular immunity in patients with RA may result in a relatively high viral load; (b) patients with RA may be more prone to infection/reactivation. The usefulness of monitoring the DNA viral load in patients with RA is questioned by these data.
确定在类风湿关节炎（RA）患者的血清和外周血单个核细胞（PBMC）中是否可检测到人疱疹病毒，即巨细胞病毒（CMV）、爱泼斯坦-巴尔病毒（EBV）和人疱疹病毒6型（HHV-6）。
通过定量实时聚合酶链反应分析133份PBMC样本（61例RA患者，72例健康供者）和136份血清样本（59例RA患者，77例健康供者）中HHV-6、EBV和CMV的DNA流行率和病毒载量。
对于PBMC样本，EBV在DNA流行率（RA患者中为56%，对照组中为33%，p = 0.009）和病毒载量（每微克DNA的拷贝数，RA患者为0 - 592.3，对照组为0 - 40.4，p = 0.001）方面存在显著差异。对于血清样本，HHV-6 DNA流行率（RA患者中为10%，对照组中为0%，p = 0.006）和病毒载量（每微克DNA的拷贝数，RA患者为0 - 529.1，对照组为0，p = 0.007）存在显著差异。
疱疹病毒可能在RA中起作用，尽管也可能有其他解释：（a）RA患者的细胞免疫缺陷可能导致相对较高的病毒载量；（b）RA患者可能更容易发生感染/病毒再激活。这些数据对监测RA患者DNA病毒载量的实用性提出了质疑。
