Korobowicz E, Zdunek M
Pol J Pathol. 2000;51(2):71-6.
The purpose of the study was to assess the expression of p53 in non-small cell lung cancer (NSCLC) before and after treatment with cisplatin and vepeside (PE) and to define a relationship between p53 expression and responsiveness to chemotherapy prior to surgery. Material for study consisted of specimens obtained from neoplastic infiltrate before chemotherapy (biopsy material) and tumour specimens obtained after chemotherapy (surgical material). The study population was a group of 35 patients with stage IIIA NSCLC. p53 protein accumulation was detected by immunohistochemistry using antibodies against p53: NCL-p53 (clone BP-53-12) (Novocastra) on paraffin embedded specimens. p53 expression was found in 21 patients (60%) before and after chemotherapy. In 14 patients (40%) p53 negativity was seen both in biopsy and surgical material. The level of p53 staining after chemotherapy as compared with that before treatment changed from -53 to +34. There was a mean increase by 1.52, which appeared statistically accidental (p > 0.70). There was no significant relationship between p53 expression and responsiveness to chemotherapy (from p > 0.33 to p > 0.70) and between the magnitude of changes in p53 expression and response to chemotherapy (p > 0.39). There was also a very low correlation (r to 0.10; p > 0.50) between responsiveness to therapy and p53 negativity.
本研究的目的是评估顺铂和依托泊苷（PE）治疗前后非小细胞肺癌（NSCLC）中p53的表达情况，并确定p53表达与术前化疗反应之间的关系。研究材料包括化疗前从肿瘤浸润组织获取的标本（活检材料）和化疗后获取的肿瘤标本（手术材料）。研究对象为35例IIIA期NSCLC患者。采用抗p53抗体NCL-p53（克隆BP-53-12）（诺沃卡斯托拉公司）对石蜡包埋标本进行免疫组织化学检测，以检测p53蛋白的积累情况。化疗前后均发现21例患者（60%）有p53表达。14例患者（40%）的活检材料和手术材料中均未见p53阴性。化疗后p53染色水平与治疗前相比，从-53变为+34。平均增加1.52，这在统计学上似乎是偶然的（p>0.70）。p53表达与化疗反应之间（p>0.33至p>0.70）以及p53表达变化幅度与化疗反应之间均无显著关系（p>0.39）。治疗反应与p53阴性之间的相关性也非常低（r为0.10；p>0.50）。
