Mańdziuk Sławomir, Dudzisz-Sledź Monika, Korszeń-Pilecka Iwona, Milanowski Janusz, Wojcierowski Jacek, Korobowicz Elzbieta
Pulmonary Department, Medical University of Lublin, Lublin, Poland.
Ann Univ Mariae Curie Sklodowska Med. 2003;58(1):154-7.
Apoptosis (programmed cell death) plays a very important role in the development regulation, homeostasis maintenance as well as in the origin of many diseases, including neoplasms. This process is genetically regulated and reflected in characteristic morphological and biochemical changes taking place in cells. The process is considered to be of great significance in tumour originating and growth as well as in tumour cell response to chemotherapy. There are many genes and their products that are involved in apoptosis. The following genes: p53, bcl-2 and p21 seem to have the greatest significance. Our study aimed at evaluating p53 gene expression in non-small-cell lung cancer patients after neoadjuvant chemotherapy. We examined the tissue material from 35 patients after three-cycle inductive chemotherapy (Vepesid and Cisplatin). The material was obtained before chemotherapy during bronchofiberoscopy and four weeks after drug treatment during surgery. The control group comprised patients who had not undergone inductive chemotherapy. After deparaffinising of tissue slides, gene p53 activity using in situ hybridisation technique was evaluated. Moreover, apoptosis valuation with TUNEL method was performed. The results were documented as photographs. Gene p53 activity level was estimated using cytophotometric technique. Our study revealed significantly higher percentage of cells undergoing apoptosis and increased gene p53 activity in tumour tissue slides of patients after neoadjuvant chemotherapy.
细胞凋亡（程序性细胞死亡）在发育调控、内环境稳态维持以及包括肿瘤在内的许多疾病的发生过程中发挥着非常重要的作用。这个过程受基因调控，并反映在细胞中发生的特征性形态和生化变化上。该过程在肿瘤的发生、生长以及肿瘤细胞对化疗的反应中被认为具有重要意义。有许多基因及其产物参与细胞凋亡。以下基因：p53、bcl-2和p21似乎具有最重要的意义。我们的研究旨在评估新辅助化疗后非小细胞肺癌患者中p53基因的表达情况。我们检查了35例患者在接受三个周期诱导化疗（依托泊苷和顺铂）后的组织材料。这些材料在化疗前通过纤维支气管镜检查获取，在药物治疗四周后手术时获取。对照组包括未接受诱导化疗的患者。在组织切片脱石蜡处理后，使用原位杂交技术评估p53基因活性。此外，采用TUNEL法进行细胞凋亡评估。结果以照片记录。使用细胞光度技术估计p53基因活性水平。我们的研究显示，新辅助化疗后患者肿瘤组织切片中发生凋亡的细胞百分比显著更高，且p53基因活性增加。
