Schlegel R A, Shortman K
J Immunol. 1975 Jul;115(1):94-9.
Distinct sub-populations of B lymphocytes may be fractionated by passage through glass-bead columns, due to a temperature-independent adherence of certain cells. The relative adherence properties of AFC-progenitors from spleens of primed or unprimed CBA mice were compared with an adoptive immune assay employing as antigen the hapten NIP on the carrier polymerized flagellin, under conditions where T lymphocytes did not limit the response. The AFC-progenitors for an anti-NIP IgM response from unprimed mice, whether conventional, germ-free, or athymic were more adherent than the majority of cells in the spleen. The AFC-progenitors for an IgG response from hapten-primed mice were less adherent that the majority of spleen cells. The results indicate that there are sub-classes of AFC-progenitors, differing in surface properties and probably corresponding to differences between the "antigen-inexperienced" and the "memory" B cell populations.
由于某些细胞具有不依赖温度的黏附性，通过玻璃珠柱可以分离出不同的B淋巴细胞亚群。在T淋巴细胞不限制反应的条件下，采用载体聚合鞭毛蛋白上的半抗原NIP作为抗原的过继免疫测定法，比较了初免或未初免CBA小鼠脾脏中抗半抗原抗体产生细胞（AFC）祖细胞的相对黏附特性。未初免小鼠中产生抗NIP IgM反应的AFC祖细胞，无论是常规小鼠、无菌小鼠还是无胸腺小鼠的，都比脾脏中的大多数细胞更具黏附性。初免小鼠中产生IgG反应的AFC祖细胞比大多数脾细胞的黏附性低。结果表明，存在AFC祖细胞亚类，其表面特性不同，可能对应于“无抗原经验”和“记忆”B细胞群体之间的差异。
