Shortman K, Howard M C, Baker J A
J Immunol. 1978 Nov;121(5):2060-5.
The effect of specific and nonspecific stimuli on the cycle status of subsets of primary B lymphocytes was assessed by preinjecting donor CBA mice 1 to 2 days previously with various substances, and then incubating the isolated spleen cells with high specific activity 3H-TdR before assay. AFC-progenitor activity was assessed as a response to NIP-POL antigen, either by adoptive transfer to irradiated recipients or by cell culture. Previous studies showed these assays reflected the activity of different subsets of B cells, termed "pre-progenitors" (adoptive assay) and "direct progenitors" (culture assay). Most functional primary B cells, whether assayed in culture or by adoptive transfer, were not initially in rapid cell cycle in normal adult mice. However, nonspecific stimulation for 1 day caused NIP-specific adoptive transfer IgM AFC-progenitors to enter rapid cell cycle. This effect was independent of T cells and not related to the antigenicity of the stimulus: particulate peritoneal irritants were the most effective stimulants. In contrast to adoptive transfer results. AFC-progenitors assayed in cell culture were unaffected by nonspecific stimuli, but were activated into cell cycle by specific antigen.
通过在1至2天前给供体CBA小鼠预先注射各种物质，然后在检测前用高比活度的³H-TdR孵育分离的脾细胞，评估特异性和非特异性刺激对原代B淋巴细胞亚群细胞周期状态的影响。AFC祖细胞活性通过对NIP-POL抗原的反应来评估，方法是将其过继转移给受辐照的受体或进行细胞培养。先前的研究表明，这些检测反映了不同B细胞亚群的活性，分别称为“前祖细胞”（过继检测）和“直接祖细胞”（培养检测）。在正常成年小鼠中，大多数具有功能的原代B细胞，无论通过培养还是过继转移检测，最初都不在快速细胞周期中。然而，1天的非特异性刺激会使NIP特异性过继转移IgM AFC祖细胞进入快速细胞周期。这种效应与T细胞无关，也与刺激的抗原性无关：颗粒性腹膜刺激物是最有效的刺激剂。与过继转移结果相反，在细胞培养中检测的AFC祖细胞不受非特异性刺激的影响，但会被特异性抗原激活进入细胞周期。
