Ren G, Chen Y T
Institute of Microbiology, Chinese Academy of Sciences, Beijing.
Sheng Wu Gong Cheng Xue Bao. 2000 Mar;16(2):198-202.
Candida tropicalis strain UH-2-48 can increase the production of decane 1,10-dicarboxylic acid(DC12) yield by 30% (from 81.7 g/L to 108.2 g/L) after regulating the fermentation condition. The purity of the DC12 is above 97%, which can meet the criteria of the succeeding synthesized industrial productions. Emulsifier can not only destroy the structure of the cell membrane in some degree, but also can enhance the permeability of the cell membrane. Furthermore, the structure of the emulsifier itself can also effect its function. Tween 60(0.10%) can enhance the production of DC12 tremendously. Urea, as the nitrogen resource, can affect the activity of cytochrome P450 enzyme. Some biochemistry and biophysics factors, such as penicillin, vitamin B2 and alanine, can increase the yield of DC12 during the process of fermentation. Ferment in 20 m3 fermentator, under the optimal condition, the average yield of DC12 using the strain HP-12 which derived from UH-2-48, is 202.1 g/L.
热带假丝酵母菌株UH-2-48在调节发酵条件后，癸烷1,10-二羧酸（DC12）产量可提高30%（从81.7克/升提高到108.2克/升）。DC12的纯度高于97%，可满足后续合成工业生产的标准。乳化剂不仅能在一定程度上破坏细胞膜结构，还能增强细胞膜的通透性。此外，乳化剂本身的结构也会影响其功能。吐温60（0.10%）能极大地提高DC12的产量。尿素作为氮源，会影响细胞色素P450酶的活性。一些生物化学和生物物理因素，如青霉素、维生素B2和丙氨酸，在发酵过程中可提高DC12的产量。在20立方米发酵罐中发酵，在最佳条件下，源自UH-2-48的菌株HP-12的DC12平均产量为202.1克/升。
