Yang J L, Gui Y L, Guo Z C
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
Sheng Wu Gong Cheng Xue Bao. 2000 Mar;16(2):218-20.
Embryogenic calli were produced from mature zygotic embryos of Picea meyeri Rehd. et Wils. which were cultured on modified LP medium containing 30 g/L sucrose, 2 mg/L 2,4-D and 1 mg/L 6-BA. The growth rate of embryogenic calli could be improved by way of suspension culture in medium MS containing 20 g/L sucrose, 1 mg/L 2,4-D and 1 mg/L KT. The optimum values of initial cell density and shake rate of flask in suspension culture were 3.0% (fresh weight) and 150 r/min respectively. Several parameters which might be correlated with growth were determined over a 10 day period. Maximum growth in terms of fresh weight, dry weight and number of ESM occurred during 6-10 day. pH and conductivity attained the lowest point during 6-8 day. A lot of somatic embryos were obtained following transfer of the suspension cultures to medium MS containing 5 mg/L ABA. Mature somatic embryos grew into plantlets with well developed cotyledons after being cultured on 1/2 MS medium supplemented with 0.5% activated charcoal but without hormone.
胚性愈伤组织由白杄成熟合子胚诱导产生，将其接种于添加了30 g/L蔗糖、2 mg/L 2,4 - D和1 mg/L 6 - BA的改良LP培养基上。通过在添加了20 g/L蔗糖、1 mg/L 2,4 - D和1 mg/L KT的MS培养基中进行悬浮培养，可提高胚性愈伤组织的生长速率。悬浮培养中初始细胞密度和摇床转速的最佳值分别为3.0%（鲜重）和150 r/min。在10天的时间内测定了几个可能与生长相关的参数。鲜重、干重和胚性体细胞团数量的最大增长出现在第6 - 10天。pH值和电导率在第6 - 8天达到最低点。将悬浮培养物转移至添加5 mg/L ABA的MS培养基后，获得了大量体细胞胚。成熟体细胞胚在添加0.5%活性炭但无激素的1/2 MS培养基上培养后，长成了子叶发育良好的小植株。
