Ahmed K Z, Sági F
Cereal Research Institute, Szeged, Hungary.
Acta Biol Hung. 1993;44(4):421-32.
Highly embryogenic cell suspension cultures were established from immature embryo-derived embryogenic calli of winter wheat (Triticum aestivum L., cv. GK Ságvári). Weekly subcultures were made in liquid MS medium supplemented with 2 mg 1(-1) 2,4-D. An average of twenty-two compact, organized calli were obtained from each 1 ml suspension cells when plated on solid MS medium containing IAA and zeatin under a 16/8 h light/dark cycle, while only 9 calli were produced in the dark. Variation in the callus inducing ability was correlated to the time elapsed after subculture. Plated cells responded best 9 days after the subculture and 59% of the calli were regenerable, retaining their embryogenic capacity over 6 months. Several hundred green shoots and plants were regenerated on differentiation media supplemented with IAA and BAP, and 300 plants were transferred to the greenhouse. The majority of plants had an abnormal chromosome number and a low viability.
从冬小麦（Triticum aestivum L.，品种GK Ságvári）未成熟胚来源的胚性愈伤组织中建立了高胚性细胞悬浮培养物。每周在添加了2 mg/L 2,4-D的液体MS培养基中进行继代培养。当将每1 ml悬浮细胞接种在含有IAA和玉米素的固体MS培养基上，在16/8小时光照/黑暗周期下培养时，平均从每个培养物中获得22个紧密、有组织的愈伤组织，而在黑暗中仅产生9个愈伤组织。愈伤组织诱导能力的变化与继代培养后经过的时间相关。接种的细胞在继代培养9天后反应最佳，59%的愈伤组织可再生，并且在6个月内保持其胚性能力。在添加了IAA和BAP的分化培养基上再生了数百株绿苗和植株，300株植株被转移到温室中。大多数植株染色体数目异常且活力较低。
