紫外线照射对大肠杆菌噬菌体T1转导的影响。
Effect of UV irradiation on transduction by coliphage T1.
作者信息
Drexler H, Kylberg K J
出版信息
J Virol. 1975 Aug;16(2):263-6. doi: 10.1128/JVI.16.2.263-266.1975.
Lysates of the virulent coliphage T1 transduce seven markers between strains of Escherichia coli with reproducilble efficiencies which range from 10(-7) to 10(-5). The ability of a UV-irradiated lysate to transduce Arg(+), Str(R), Trp(+), Lac(+), and Pro(+) is 90% and Bio(+) is 99% inactivated by doses which inactivate plaque formation of T1 by six orders of magnitude. A dose of irradiation which causes a 1- to 2-log drop in the titer of T1 stimulated Gal(+) transduction by two- to three-fold; no other marker tested was stimulated. Irradiation causes dislinkage of some co-transducible markers but not others.
烈性大肠杆菌噬菌体T1的裂解物能在大肠杆菌菌株间转导7个标记，转导效率可重复，范围在10^(-7)至10^(-5)之间。紫外线照射的裂解物转导精氨酸（+）、链霉素抗性（Str(R)）、色氨酸（+）、乳糖（+）和脯氨酸（+）的能力，在使T1噬菌斑形成失活六个数量级的剂量下，90%被灭活，而生物素（+）为99%。导致T1效价下降1至2个对数的照射剂量，使半乳糖（+）转导增加了两到三倍；其他测试的标记均未受到刺激。照射会导致一些共转导标记的解离，但其他标记不会。
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