Engelberg H, Brudo I, Israeli-Reches M
J Virol. 1975 Aug;16(2):340-7. doi: 10.1128/JVI.16.2.340-347.1975.
Rifampin interferes exclusively with RNA replication in vivo of the group I phages MS2, f2, and R17, whereas QbetaRNA replication is unaffected by the drug. In addition, rifampin has a discriminative effect of group I phage RNA replication. In the experimental system employed by us the antibiotic differentially interferes with the synthesis of minus RNA strands in f2, whereas it has almost no effect on the synthesis of progeny plus strands. In MS2, the drug differentially arrests the synthesis of progeny plus strands and almost fails to affect the synthesis of minus RNA strands. In R17 both steps of its RNA replication are affected by rifampin, although each step is only partially (approximately 50%) inhibited. The relation of the present results to the possible role of bacterial proteins and tertiary structure of phage RNA in the process of template recognition is discussed.
利福平仅在体内干扰I组噬菌体MS2、f2和R17的RNA复制，而QβRNA复制不受该药物影响。此外，利福平对I组噬菌体RNA复制具有鉴别作用。在我们采用的实验系统中，该抗生素对f2中负链RNA的合成有差异干扰作用，而对后代正链的合成几乎没有影响。在MS2中，该药物差异地阻止后代正链的合成，几乎不影响负链RNA的合成。在R17中，其RNA复制的两个步骤都受到利福平的影响，尽管每个步骤仅受到部分（约50%）抑制。本文讨论了这些结果与细菌蛋白及噬菌体RNA三级结构在模板识别过程中可能作用的关系。
