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两种血清学上不同的核糖核酸噬菌体的比较。I. 病毒颗粒的特性。

Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of the viral particles.

作者信息

Overby L R, Barlow G H, Doi R H, Jacob M, Spiegelman S

出版信息

J Bacteriol. 1966 Jan;91(1):442-8. doi: 10.1128/jb.91.1.442-448.1966.

DOI:10.1128/jb.91.1.442-448.1966
PMID:5903109
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC315966/
Abstract

Overby, L. R. (University of Illinois, Urbana), G. H. Barlow, R. H. Doi, Monique Jacob, and S. Spiegelman. Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of the viral particle. J. Bacteriol. 91:442-448. 1966.-Two ribonucleic acid (RNA) coliphages, MS-2 and Qbeta, have been characterized physically and serologically. MS-2 has an S(20, w) value of 79, a molecular weight of 3.6 x 10(6), a density of 1.422, and pH 3.9 as its isoelectric point. Qbeta has an S(20, w) of 84, a molecular weight of 4.2 x 10(6), a density of 1.439, and an isoelectric point at pH 5.3. One host (Escherichia coli A-19) permits a distinction between the two on the basis of a marked difference in plaque size. They are distinct immunochemically, no serological cross-reaction being detectable.

摘要

奥弗比,L.R.(伊利诺伊大学厄巴纳分校),G.H.巴洛,R.H.多伊,莫妮克·雅各布,和S.斯皮格尔曼。两种血清学上不同的核糖核酸噬菌体的比较。I.病毒颗粒的特性。《细菌学杂志》91:442 - 448。1966年。——两种核糖核酸(RNA)大肠杆菌噬菌体,MS - 2和Qβ,已通过物理和血清学方法进行了表征。MS - 2的S(20,w)值为79,分子量为3.6×10⁶,密度为1.422,等电点为pH 3.9。Qβ的S(20,w)为84,分子量为4.2×10⁶,密度为1.439,等电点为pH 5.3。一种宿主(大肠杆菌A - 19)基于噬菌斑大小的显著差异可区分这两种噬菌体。它们在免疫化学上是不同的,未检测到血清学交叉反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f6/315966/ebd1c8237c7a/jbacter00419-0481-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f6/315966/f6bb0f8c1b6e/jbacter00419-0480-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f6/315966/ea5c8356649e/jbacter00419-0481-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f6/315966/ebd1c8237c7a/jbacter00419-0481-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f6/315966/f6bb0f8c1b6e/jbacter00419-0480-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f6/315966/ea5c8356649e/jbacter00419-0481-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/35f6/315966/ebd1c8237c7a/jbacter00419-0481-b.jpg

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