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[使用塑料“特法恩”进行细胞培养的维持和研究]

[Use of the plastic "Terphane" for the maintenance and the study of cell cultures].

作者信息

Gueguen C, Guillouzo A, Latinier M, Lenoir P

出版信息

Pathol Biol (Paris). 1975 Apr;23(4):333-8.

PMID:1097996
Abstract

Terphane plastic used as substrate for tissue culture, facilitates both cytochemcial, biochemical and ulstratructural studies of cells in situ. Particularly pliable, thin and resistant, the sheet of Terphane plastic is held tense by a system of two plaques of Petri dish type. This apparatus is perfectly waterproof, sterilisable and may be used indefinitely. Growth, morphology, ultrastructure and enzyme activities of primary or subcultures of rat and human liver maintained on terphane plastic are comparable with those of cells cultivated on Falcon polystyrene. Furthermore, terphane offers several advantages over the Melinex plastic: its thinness, resistance and plasticity render easy all manipulations.

摘要

用作组织培养底物的特芬塑料有助于对原位细胞进行细胞化学、生物化学和超微结构研究。特芬塑料片特别柔韧、轻薄且耐用,由两个培养皿型平板系统固定绷紧。该装置完全防水、可消毒且可无限期使用。在特芬塑料上培养的大鼠和人肝脏原代或传代培养物的生长、形态、超微结构和酶活性与在Falcon聚苯乙烯上培养的细胞相当。此外,与梅林克斯塑料相比,特芬塑料具有几个优点:其轻薄、耐用和可塑性使所有操作都很容易。

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