对由前庭蜗神经刺激诱发的小鼠脑干进行多部位光学记录。

Multiple-site optical recording of mouse brainstem evoked by vestibulocochlear nerve stimulation.

作者信息

Yang S, Doi T, Asako M, Matsumoto-Ono A, Kaneko T, Yamashita T

机构信息

Department of Otolaryngology, Kansai Medical University, Fumizono-cho 10-15, Moriguchi, 570-8507, Osaka, Japan.

出版信息

Brain Res. 2000 Sep 15;877(1):95-100. doi: 10.1016/s0006-8993(00)02659-7.

DOI:10.1016/s0006-8993(00)02659-7

PMID:10980249

Abstract

We used optical imaging to investigate the mouse cochlear and vestibular nucleus in brainstem slices using a voltage-sensitive dye, RH 155. As a result, the spatiotemporal patterns of excitatory propagation were shown. These optical signals consisted of two components consisting of a spike-like fast signal and a long-lasting slow signal. All responses were abolished by tetrodotoxin. The slow signals were eliminated under a Ca(2+)-free solution. In addition, synaptic fatigue was also observed. The present study indicated the feasibility of optical recording for visually revealing the synaptic transmission in both the vestibular and cochlear nucleus.

摘要

我们使用光学成像技术，利用电压敏感染料RH 155对脑干切片中的小鼠耳蜗和前庭核进行研究。结果显示了兴奋性传播的时空模式。这些光学信号由两个成分组成，一个是尖峰状的快速信号，另一个是持久的慢速信号。所有反应都被河豚毒素消除。在无钙溶液中，慢速信号消失。此外，还观察到了突触疲劳。本研究表明光学记录在直观揭示前庭核和耳蜗核中的突触传递方面具有可行性。