酶联免疫吸附测定法在澳大利亚兔出血性疾病（RHD）现场研究中的应用。
Use of ELISAs in field studies of rabbit haemorrhagic disease (RHD) in Australia.
作者信息
Cooke B D, Robinson A J, Merchant J C, Nardin A, Capucci L
机构信息
CSIRO, Wildlife and Ecology, Canberra, ACT, Australia.
出版信息
Epidemiol Infect. 2000 Jun;124(3):563-76. doi: 10.1017/s0950268899003994.
ELISA techniques developed for the veterinary diagnosis of Rabbit Haemorrhagic Disease (RHD) in domestic rabbits were used for studying the epidemiology of RHD in Australian wild rabbits. The combination of ELISA techniques that distinguished IgA, IgG and IgM antibody responses and a longitudinal data set, mainly based on capture-mark-recapture of rabbits, provided a reliable basis for interpreting serology and set the criteria used to classify rabbits' immunological status. Importantly, young with maternal antibodies, immune rabbits and rabbits apparently re-exposed to RHD were readily separated. Three outbreaks of RHD occurred in 1996-7. The timing of RHD outbreaks was mainly driven by recruitment of young rabbits that generally contracted RHD after they lost their maternally derived immunity. Young that lost maternal antibodies in summer were not immediately infected, apparently because transmission of RHDV slows at that time, but contracted RHD in the autumn when conditions were again suitable for disease spread.
为家兔兔出血症（RHD）的兽医诊断而开发的酶联免疫吸附测定（ELISA）技术，被用于研究澳大利亚野兔中RHD的流行病学。区分IgA、IgG和IgM抗体反应的ELISA技术与主要基于对兔子进行标记重捕的纵向数据集相结合，为解释血清学提供了可靠依据，并设定了用于分类兔子免疫状态的标准。重要的是，带有母源抗体的幼兔、免疫兔和明显再次接触RHD的兔子很容易区分开来。1996 - 1997年发生了三次RHD疫情。RHD疫情的发生时间主要由幼兔的补充数量驱动，这些幼兔通常在失去母源免疫力后感染RHD。在夏季失去母源抗体的幼兔不会立即被感染，显然是因为那时RHD病毒的传播速度减缓，但在秋季条件再次适合疾病传播时感染了RHD。
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