Perova E V, Golikov V I, Bulatova T I, Il'iashenko B N
Zh Mikrobiol Epidemiol Immunobiol. 1975 Jun(6):89-92.
A possibility of detection of atoxigenic clones in the cultures of Cl. botulinum, types A and B, under conditions of treatment with acridine strains, UV-irradiation and heating of spores was studied. Of the 10 strains investigated, atoxigenic variants were obtained only in 3, belonging to type B; the frequency of formation of atoxigenic clones under the effect of ethidium bronide constituted 36--45.7%, and of the UV-irradiation-4.2%; heating of spores proved to be ineffective. A frequent loss of the toxigenicity sign coincided with the view on the elimination of the episomal determinant of the toxin-formation, not with the mutational nature-of this change. An association between the differences in stability of retention of the toxigenicity signs in various strains and types of Cl. botulinum and possible differences of the corresponding genetic determinants of the toxin formation is discussed.
研究了在吖啶橙处理、紫外线照射和孢子加热条件下，检测A型和B型肉毒梭菌培养物中无毒素产生克隆的可能性。在所研究的10株菌株中，仅在3株B型菌株中获得了无毒素产生的变体；在溴化乙锭作用下无毒素产生克隆的形成频率为36%-45.7%，紫外线照射下为4.2%；孢子加热被证明是无效的。毒素产生特性的频繁丧失与关于消除毒素形成的附加体决定因素的观点一致，而与这种变化的突变性质无关。讨论了肉毒梭菌不同菌株和类型中毒素产生特性保留稳定性的差异与毒素形成相应遗传决定因素可能差异之间的关联。
