Tatakis D N, Wikesjö U M, Razi S S, Sigurdsson T J, Lee M B, Nguyen T, Ongpipattanakul B, Hardwick R
Loma Linda University, Advanced Education Program in Periodontics, CA 92354, USA.
J Clin Periodontol. 2000 Sep;27(9):698-704. doi: 10.1034/j.1600-051x.2000.027009698.x.
Transforming growth factor-beta (TGF-beta) represents a family of growth-modulating proteins with fundamental roles in connective tissue and bone development. The objective of this study was to evaluate the potential for regeneration of alveolar bone and cementum following surgical implantation of recombinant human TGF-beta 1 (rhTGF-beta 1).
Bilateral, critical size, supra-alveolar periodontal defects in 5 beagle dogs were surgically implanted with rhTGF-beta 1 in a calcium carbonate carrier (CaCO3) or with carrier alone. The animals were euthanized at 4 weeks postsurgery and block-biopsies of the defects were processed for histologic and histometric analysis.
Surgical implantation of rhTGF-beta 1 resulted in minimal, if any, stimulation of alveolar bone or cementum regeneration. Linear bone and cementum regeneration in rhTGF-beta 1-treated defects was 1.2+/-0.6 and 0.01+0.01 mm, respectively. Corresponding values for the controls were 1.0+/-0.6 and 0.01+/-0.03 mm.
The results suggest that, under the conditions (dose, carrier, defect type) evaluated here, treatment of periodontal defects in beagle dogs with rhTGF-beta 1 may be of limited clinical benefit.
转化生长因子-β（TGF-β）是一类生长调节蛋白家族，在结缔组织和骨骼发育中起重要作用。本研究的目的是评估重组人TGF-β1（rhTGF-β1）手术植入后牙槽骨和牙骨质再生的潜力。
对5只比格犬双侧造成临界大小的牙槽上牙周缺损，一侧手术植入rhTGF-β1于碳酸钙载体（CaCO3）中，另一侧仅植入载体。术后4周对动物实施安乐死，对缺损处进行整块活检，进行组织学和组织计量学分析。
rhTGF-β1手术植入后，对牙槽骨或牙骨质再生的刺激作用极小（若有）。rhTGF-β1处理的缺损处线性骨和牙骨质再生分别为1.2±0.6和0.01±0.01毫米。对照组的相应值分别为1.0±0.6和0.01±0.03毫米。
结果表明，在此处评估的条件（剂量、载体、缺损类型）下，用rhTGF-β1治疗比格犬的牙周缺损临床益处可能有限。
