Beck C F
Proc Natl Acad Sci U S A. 1979 May;76(5):2376-80. doi: 10.1073/pnas.76.5.2376.
Integration of the tetracycline resistance transposon Tn10 into lacI of a lacI-lacZ gene fusion permits the isolation of deletions that excise DNA from one end of Tn10 and fuse Tn10 genes with lacZ in such a manner that chimeric proteins with beta-galactosidase activity are produced. The synthesis of the chimeric proteins is under the same control as the transposon genes. Thus, regulation of expression of Tn10 genes can be investigated by measuring beta-galactosidase activity. Analysis of Tn10-lacZ fusions revealed different deletion endpoints within Tn10; lacZ has been fused to at least three different Tn10 genes or operons. Two of these genes are under the control of a tetracycline repressor.
四环素抗性转座子Tn10整合到lacI-lacZ基因融合体的lacI中，可分离出从Tn10一端切除DNA的缺失突变体，并使Tn10基因与lacZ融合，从而产生具有β-半乳糖苷酶活性的嵌合蛋白。嵌合蛋白的合成与转座子基因受相同的调控。因此，可通过测量β-半乳糖苷酶活性来研究Tn10基因的表达调控。对Tn10-lacZ融合体的分析揭示了Tn10内不同的缺失端点；lacZ已与至少三个不同的Tn10基因或操纵子融合。其中两个基因受四环素阻遏物的控制。
