Way J C, Davis M A, Morisato D, Roberts D E, Kleckner N
Gene. 1984 Dec;32(3):369-79. doi: 10.1016/0378-1119(84)90012-x.
We describe below several new variants of the tetracycline-resistance transposon Tn10 which are more useful than the wild-type transposon for many types of genetic and physical analysis of bacteria. These derivatives have one or more of the following new properties: (i) new drug resistance markers; (ii) high transposition frequencies; (iii) removal of the transposase gene to a position outside of the transposing segment; (iv) internal deletions which eliminate the ability of Tn10 to make adjacent deletion/inversions; or (v) addition of a trp-lac operon fusion segment just inside one terminus such that insertion can automatically generate a transcriptional fusion to the interrupted operon. Phage and plasmid vehicles carrying these new elements are described.
我们在下面描述了四环素抗性转座子Tn10的几种新变体，对于细菌的多种遗传和物理分析而言，这些变体比野生型转座子更有用。这些衍生物具有以下一种或多种新特性：（i）新的耐药标记；（ii）高转座频率；（iii）将转座酶基因移至转座片段之外的位置；（iv）内部缺失，消除了Tn10产生相邻缺失/倒位的能力；或（v）在一个末端内侧添加一个trp-lac操纵子融合片段，使得插入能够自动产生与被中断操纵子的转录融合。本文还描述了携带这些新元件的噬菌体和质粒载体。
