Collavin L, Monte M, Verardo R, Pfleger C, Schneider C
Laboratorio Nazionale Consorzio Interuniversitario Biotechnologie, Trieste, Italy.
FEBS Lett. 2000 Sep 8;481(1):57-62. doi: 10.1016/s0014-5793(00)01969-4.
B99 is a p53-inducible gene whose accumulation upon p53 activation is restricted to late S/G2 cells. Here we have analyzed B99 regulation during the cell cycle in murine cells with or without functional p53. We report that B99 accumulates in late S/G2 phase, is phosphorylated in mitosis, and disappears in G1 phase, regardless of the status of p53. As a complement to this observation, we show that B99 is not induced by p53 in quiescent cells. Therefore, B99 expression is modulated both by cell-cycle regulatory mechanisms and by p53, and p53 can increase the cellular levels of B99 only during the window of the cell cycle when it is normally expressed. On the basis of these observations we rename B99 Gtse-1 (G-two- and S-phase-expressed).
B99是一种p53诱导基因，其在p53激活后在S/G2晚期细胞中积累。在此，我们分析了在有或没有功能性p53的鼠细胞中，细胞周期内B99的调控情况。我们报告称，无论p53状态如何，B99都在S/G2晚期积累，在有丝分裂期被磷酸化，并在G1期消失。作为这一观察结果的补充，我们表明在静止细胞中B99不会被p53诱导。因此，B99的表达受细胞周期调控机制和p53的双重调节，并且p53仅能在其正常表达的细胞周期窗口内增加B99的细胞水平。基于这些观察结果，我们将B99重新命名为Gtse-1（G2期和S期表达）。
