Nishimori Y, Lee J M, Sumitani M, Hatakeyama M, Oishi K
Graduate School of Science and Technology, Kobe University, Japan.
Genes Genet Syst. 2000 Jun;75(3):159-66. doi: 10.1266/ggs.75.159.
A linkage map was constructed for the sawfly, Athalia rosae (Hymenoptera), based on the segregation of random amplified polymorphic DNA (RAPD) markers and a visible mutation, yellow fat body (yfb). Forty haploid male progeny (20 yfb and 20+) from a single diploid female parent (yfb/+) were examined. Sixty-one of the 180 arbitrary primers tested by polymerase chain reaction (PCR) produced one or more RAPD bands. A total of 79 RAPD markers were detected. Of these, seven showed significant deviation from the expected 1:1 ratio, and were therefore excluded from further analysis. The remaining 72 RAPD markers and the marker mutation, yfb, were subjected to linkage analysis. Sixty RAPD markers and the yfb marker were organized into 16 linkage groups, spanning a distance of 517.2 cM. Twelve RAPD markers showed no linkage relationship to any group. Thirteen gel-purified RAPD bands were cloned and sequenced to generate the sequence-tagged sites (STSs). A single locus was represented by two markers, with one of them having a short internal deletion.
基于随机扩增多态性DNA（RAPD）标记的分离和一个可见突变——黄脂肪体（yfb），构建了蔷薇叶蜂（膜翅目）的连锁图谱。对来自单个二倍体雌性亲本（yfb/+）的40个单倍体雄性后代（20个yfb和20个+）进行了检测。通过聚合酶链反应（PCR）检测的180个任意引物中，有61个产生了一个或多个RAPD条带。共检测到79个RAPD标记。其中，7个标记显示出与预期的1:1比例有显著偏差，因此被排除在进一步分析之外。其余72个RAPD标记和标记突变yfb进行了连锁分析。60个RAPD标记和yfb标记被组织成16个连锁群，跨度为517.2厘摩。12个RAPD标记与任何群体均无连锁关系。对13个凝胶纯化的RAPD条带进行了克隆和测序，以生成序列标签位点（STS）。一个位点由两个标记表示，其中一个有短的内部缺失。
