基于随机扩增多态性DNA（RAPD）标记的蜜蜂（西方蜜蜂）连锁图谱。
Linkage map of the honey bee, Apis mellifera, based on RAPD markers.
作者信息
Hunt G J, Page R E
机构信息
Department of Entomology, University of California, Davis 95616, USA.
出版信息
Genetics. 1995 Mar;139(3):1371-82. doi: 10.1093/genetics/139.3.1371.
Abstract
A linkage map was constructed for the honey bee based on the segregation of 365 random amplified polymorphic DNA (RAPD) markers in haploid male progeny of a single female bee. The X locus for sex determination and genes for black body color and malate dehydrogenase were mapped to separate linkage groups. RAPD markers were very efficient for mapping, with an average of about 2.8 loci mapped for each 10-nucleotide primer that was used in polymerase chain reactions. The mean interval size between markers on the map was 9.1 cM. The map covered 3110 cM of linked markers on 26 linkage groups. We estimate the total genome size to be approximately 3450 cM. The size of the map indicated a very high recombination rate for the honey bee. The relationship of physical to genetic distance was estimated at 52 kb/cM, suggesting that map-based cloning of genes will be feasible for this species.
摘要
基于一只雌蜂单倍体雄性后代中365个随机扩增多态性DNA（RAPD）标记的分离情况，构建了蜜蜂的连锁图谱。用于性别决定的X位点以及黑体色和苹果酸脱氢酶的基因被定位到不同的连锁群上。RAPD标记在图谱构建中非常有效，在聚合酶链反应中使用的每个10核苷酸引物平均定位约2.8个位点。图谱上标记之间的平均间隔大小为9.1厘摩（cM）。该图谱覆盖了26个连锁群上3110 cM的连锁标记。我们估计蜜蜂的基因组总大小约为3450 cM。图谱的大小表明蜜蜂具有非常高的重组率。物理距离与遗传距离的关系估计为52 kb/cM，这表明基于图谱的基因克隆对于该物种是可行的。
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