RIC-8（Synembryn）：一种新型保守蛋白，是秀丽隐杆线虫神经系统中G（q）α信号传导所必需的。
RIC-8 (Synembryn): a novel conserved protein that is required for G(q)alpha signaling in the C. elegans nervous system.
作者信息
Miller K G, Emerson M D, McManus J R, Rand J B
机构信息
Program in Molecular and Cell Biology, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
出版信息
Neuron. 2000 Aug;27(2):289-99. doi: 10.1016/s0896-6273(00)00037-4.
Abstract
Recent studies describe a network of signaling proteins centered around G(o)alpha and G(q)alpha that regulates neurotransmitter secretion in C. elegans by controlling the production and consumption of diacylglycerol (DAG). We sought other components of the Goalpha-G(q)alpha signaling network by screening for aldicarb-resistant mutants with phenotypes similar to egl-30 (G(q)alpha) mutants. In so doing, we identified ric-8, which encodes a novel protein named RIC-8 (synembryn). Through cDNA analysis, we show that RIC-8 is conserved in vertebrates. Through immunostaining, we show that RIC-8 is concentrated in the cytoplasm of neurons. Exogenous application of phorbol esters or loss of DGK-1 (diacylglycerol kinase) rescues ric-8 mutant phenotypes. A genetic analysis suggests that RIC-8 functions upstream of, or in conjunction with, EGL-30 (G(q)alpha).
摘要
最近的研究描述了一个以G(o)α和G(q)α为中心的信号蛋白网络，该网络通过控制二酰基甘油（DAG）的产生和消耗来调节秀丽隐杆线虫中的神经递质分泌。我们通过筛选具有与egl-30（G(q)α）突变体相似表型的抗涕灭威突变体，寻找Goα-G(q)α信号网络的其他成分。在此过程中，我们鉴定出ric-8，它编码一种名为RIC-8（synembryn）的新型蛋白质。通过cDNA分析，我们表明RIC-8在脊椎动物中是保守的。通过免疫染色，我们表明RIC-8集中在神经元的细胞质中。佛波酯的外源应用或DGK-1（二酰基甘油激酶）的缺失可挽救ric-8突变体表型。遗传分析表明，RIC-8在EGL-30（G(q)α）的上游起作用或与之协同作用。
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本文引用的文献
1
Egg-laying defective mutants of the nematode Caenorhabditis elegans.线虫秀丽隐杆线虫的产卵缺陷突变体。
Genetics. 1983 Aug;104(4):619-47. doi: 10.1093/genetics/104.4.619.
2
Serotonin inhibition of synaptic transmission: Galpha(0) decreases the abundance of UNC-13 at release sites.血清素对突触传递的抑制作用：Gα(0)降低释放位点处UNC-13的丰度。
Neuron. 1999 Sep;24(1):231-42. doi: 10.1016/s0896-6273(00)80835-1.
3
Facilitation of synaptic transmission by EGL-30 Gqalpha and EGL-8 PLCbeta: DAG binding to UNC-13 is required to stimulate acetylcholine release.EGL-30 Gqα和EGL-8 PLCβ对突触传递的促进作用：二酰基甘油（DAG）与UNC-13结合是刺激乙酰胆碱释放所必需的。
Neuron. 1999 Oct;24(2):335-46. doi: 10.1016/s0896-6273(00)80848-x.
4
Goalpha and diacylglycerol kinase negatively regulate the Gqalpha pathway in C. elegans.Goα和二酰基甘油激酶对秀丽隐杆线虫中的Gqα信号通路起负调控作用。
Neuron. 1999 Oct;24(2):323-33. doi: 10.1016/s0896-6273(00)80847-8.
5
Antagonism between G(o)alpha and G(q)alpha in Caenorhabditis elegans: the RGS protein EAT-16 is necessary for G(o)alpha signaling and regulates G(q)alpha activity.秀丽隐杆线虫中G(o)α与G(q)α之间的拮抗作用：RGS蛋白EAT-16对G(o)α信号传导至关重要并调节G(q)α活性。
Genes Dev. 1999 Jul 15;13(14):1780-93. doi: 10.1101/gad.13.14.1780.
6
The cat-1 gene of Caenorhabditis elegans encodes a vesicular monoamine transporter required for specific monoamine-dependent behaviors.秀丽隐杆线虫的cat-1基因编码一种特定单胺依赖行为所需的囊泡单胺转运体。
J Neurosci. 1999 Jan 1;19(1):72-84. doi: 10.1523/JNEUROSCI.19-01-00072.1999.
7
Regulation of the readily releasable vesicle pool by protein kinase C.蛋白激酶C对易释放囊泡池的调节
Neuron. 1998 Oct;21(4):885-93. doi: 10.1016/s0896-6273(00)80603-0.
8
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans.双链RNA在秀丽隐杆线虫中产生强大而特异的基因干扰作用。
Nature. 1998 Feb 19;391(6669):806-11. doi: 10.1038/35888.
9
Molecules in focus: diacylglycerol kinase.聚焦分子：二酰基甘油激酶
Int J Biochem Cell Biol. 1997 Oct;29(10):1139-43. doi: 10.1016/s1357-2725(97)00037-x.
10
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.空位BLAST和位置特异性迭代BLAST：新一代蛋白质数据库搜索程序。
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402. doi: 10.1093/nar/25.17.3389.
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