C6大鼠胶质瘤细胞中二肽基肽酶IV的异质性。

Heterogeneity of dipeptidyl peptidase IV from C6 rat glioma cells.

作者信息

Malík R, Vlasicová L, Kadlecová L, Sedo A

机构信息

1st Department of Medical Chemistry and Biochemistry, 1st Faculty of Medicine, Charles University, Prague 2, Czech Republic.

出版信息

Neoplasma. 2000;47(2):96-9.

PMID:10985474

Abstract

Dipeptidyl peptidase IV is known to be involved, due to both hydrolytic and non-hydrolytic mechanisms, in various cell functions of normal and cancer cells as well. In this report dipeptidyl peptidase IV substrate and pH preferences, some inhibition parameters, freezing/thawing sensitivity and stability against hydrolysis by trypsin were studied in C6 rat glioma cells. Our results confirmed substantial heterogeneity of dipeptidyl peptidase IV population. Such observation is important to avoid methodological artifacts and to decrease risk of misinterpretations in biological studies.

摘要

已知二肽基肽酶IV通过水解和非水解机制参与正常细胞和癌细胞的各种细胞功能。在本报告中，研究了C6大鼠胶质瘤细胞中二肽基肽酶IV的底物和pH偏好、一些抑制参数、冻融敏感性以及对胰蛋白酶水解的稳定性。我们的结果证实了二肽基肽酶IV群体存在显著的异质性。这种观察对于避免方法学假象以及降低生物学研究中错误解读的风险很重要。