Malík R, Mares V, Kleibl Z, Pohlreich P, Vlasicová K, Sedo A
Joint Laboratory of Cancer Cell Biology, First Department, First Faculty of Medicine, Katerinská 32, Prague 2, CZ-12108, Czech Republic.
Biochem Biophys Res Commun. 2001 Jun 8;284(2):289-94. doi: 10.1006/bbrc.2001.4956.
Attractin/mahogany protein was previously shown to be involved in a number of physiological and pathological events, including immune system regulation, body weight control, pigmentation, myelinization, and tumor susceptibility. Human attractin has an enzymatic activity resembling dipeptidyl peptidase IV (DPP-IV). In the central nervous system, attractin has been detected in neurons but not in glial cells up to now. We show the expression of attractin mRNA and protein in glioma cell lines at different degree of transformation. In human U373 and U87 glioma cells (Grades III and IV), membrane-bound attractin displays hydrolytic activity amounting to 5 and 25% of total cellular DPP-IV-like enzyme activity, respectively. Such activity has not been observed in the rat C6 glioma cells (Grade I). Attractin presence in glioma, but not in normal glial cells, together with its differential enzymatic activity, suggests its role in growth properties of tumors of glial cell origin.
吸引素/红木蛋白先前已被证明参与了许多生理和病理过程，包括免疫系统调节、体重控制、色素沉着、髓鞘形成以及肿瘤易感性。人吸引素具有类似于二肽基肽酶IV（DPP-IV）的酶活性。在中枢神经系统中，到目前为止，吸引素已在神经元中检测到，但在神经胶质细胞中未检测到。我们展示了吸引素mRNA和蛋白在不同转化程度的胶质瘤细胞系中的表达。在人U373和U87胶质瘤细胞（III级和IV级）中，膜结合的吸引素分别显示出相当于总细胞DPP-IV样酶活性5%和25%的水解活性。在大鼠C6胶质瘤细胞（I级）中未观察到这种活性。吸引素存在于胶质瘤中，而不存在于正常神经胶质细胞中，以及其不同的酶活性，表明它在神经胶质细胞源性肿瘤的生长特性中发挥作用。
