Heegaard S, Jensen O A, Prause J U
Eye Pathology Institute, University of Copenhagen, Denmark.
Melanoma Res. 2000 Aug;10(4):350-4. doi: 10.1097/00008390-200008000-00006.
A novel antibody A103, which recognizes melan-A/MART-1, has been found to be more sensitive than the antibody HMB-45, which recognizes gp100, in melanocytic lesions of the skin and might therefore also be useful in the diagnosis of uveal and conjunctival melanocytic lesions. In this study we compared the staining characteristics of anti-melan-A, anti-S100 protein and HMB-45 in 13 conjunctival, 11 iris and 37 ciliary and choroidal malignant melanomas. The ciliary and choroidal melanomas comprised 13 spindle cell (10 spindle B and three spindle A), 14 mixed cell and 10 epithelioid cell tumours. In the conjunctival melanomas the diagnostic sensitivity was 100% for anti-S100 and anti-melan-A and 85% for HMB-45. In the iris melanomas the sensitivity was 100% for anti-S100 and anti-melan-A and 55% for HMB-45. A high staining intensity of anti-melan-A was particularly noticed in iris melanomas. In the choroidal malignant melanomas, the spindle cell and mixed cell types showed a sensitivity of only 69-79% with all three antibodies. In the epithelioid cell type the sensitivity was 80% for anti-S100 and 100% for HMB-45 and anti-melan-A. In conclusion, anti-melan-A was found to be a useful addition to antibody panels for ocular melanocytic lesions. Anti-melan-A has a higher sensitivity than HMB-45 in conjunctival and iris melanomas, but the sensitivity is similar to HMB-45 in choroidal melanomas. Anti-melan-A stains in a very similar pattern to anti-S100, but the staining intensity of anti-melan-A is higher than that of anti-S100 in iris melanoma.
一种识别黑色素A/MART-1的新型抗体A103，已被发现在皮肤黑素细胞病变中比识别糖蛋白100的抗体HMB-45更敏感，因此也可能有助于葡萄膜和结膜黑素细胞病变的诊断。在本研究中，我们比较了抗黑色素A、抗S100蛋白和HMB-45在13例结膜、11例虹膜以及37例睫状体和脉络膜恶性黑色素瘤中的染色特征。睫状体和脉络膜黑色素瘤包括13例梭形细胞（10例梭形B和3例梭形A）、14例混合细胞和10例上皮样细胞瘤。在结膜黑色素瘤中，抗S100和抗黑色素A的诊断敏感性为100%，HMB-45为85%。在虹膜黑色素瘤中，抗S100和抗黑色素A的敏感性为100%，HMB-45为55%。抗黑色素A在虹膜黑色素瘤中的染色强度特别高。在脉络膜恶性黑色素瘤中，梭形细胞和混合细胞类型对所有三种抗体的敏感性仅为69%-79%。在上皮样细胞类型中，抗S100的敏感性为80%，HMB-45和抗黑色素A为100%。总之，抗黑色素A被发现是用于眼部黑素细胞病变抗体组合中的一种有用补充。抗黑色素A在结膜和虹膜黑色素瘤中的敏感性高于HMB-45，但在脉络膜黑色素瘤中的敏感性与HMB-45相似。抗黑色素A的染色模式与抗S100非常相似，但在虹膜黑色素瘤中抗黑色素A的染色强度高于抗S100。
