Alghamdi Sarah A, Zoroquiain Pablo, Dias Ana Beatriz T, Alhumaid Sulaiman R, Aldrees Sultan, Burnier Miguel N
Henry C Witelson Ocular Pathology Laboratory, McGill University, H4A 3J1, Canada.
Ecancermedicalscience. 2015 Aug 20;9:566. doi: 10.3332/ecancer.2015.566. eCollection 2015.
SOX-10 has been shown to be a sensitive marker of cutaneous melanoma. This study aimed to evaluate Sox-10 expression in uveal melanoma.
A total of 40 tissue blocks of enucleated eyes with uveal melanoma were cut and stained using an anti-SOX-10 mouse monoclonal antibody and HMB-45 antibody.
SOX-10 showed exclusive nuclear positivity in 100% of the uveal melanoma cases (38/38). HMB-45 showed cytoplasmic positivity in 97.3 (37/38). Positivity for SOX-10 was also noted in the inner and outer nuclear layers of the retina in 78% of the enucleated eyes.
SOX-10 expression proved to be the most sensitive marker for uveal melanoma, and therefore, we propose a modified panel for the diagnosis of uveal melanoma that includes both SOX-10 and HMB-45. The observation of distinct, diffuse nuclear SOX-10 expression in retinal inner and outer nuclear layers is a finding that warrants further investigation as a marker for retinoblastoma.
SOX - 10已被证明是皮肤黑色素瘤的敏感标志物。本研究旨在评估Sox - 10在葡萄膜黑色素瘤中的表达。
共切取40个患有葡萄膜黑色素瘤的摘除眼球组织块，并用抗SOX - 10小鼠单克隆抗体和HMB - 45抗体进行染色。
SOX - 10在100%的葡萄膜黑色素瘤病例（38/38）中显示出仅细胞核阳性。HMB - 45在97.3%（37/38）的病例中显示出细胞质阳性。在78%的摘除眼球的视网膜内核层和外核层中也观察到SOX - 10阳性。
SOX - 10表达被证明是葡萄膜黑色素瘤最敏感的标志物，因此，我们提出一种用于诊断葡萄膜黑色素瘤的改良检测组合，包括SOX - 10和HMB - 45。视网膜内核层和外核层中明显、弥漫性的细胞核SOX - 10表达这一观察结果，作为视网膜母细胞瘤的标志物值得进一步研究。
