一种使用醛缩酶抗体进行多克规模动力学拆分的高效台式系统。
An efficient benchtop system for multigram-scale kinetic resolutions using aldolase antibodies.
作者信息
Turner J M, Bui T, Lerner R A, Barbas C F, List B
机构信息
The Skaggs Institute for Chemical Biology, Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
出版信息
Chemistry. 2000 Aug 4;6(15):2772-4. doi: 10.1002/1521-3765(20000804)6:15<2772::aid-chem2772>3.0.co;2-j.
The preparative scale kinetic resolution of racemic aldols 1-4 using aldolase antibodies 38C2 (Aldrich no. 47995-0) and 84G3 (Aldrich no. 52785-8) is described. These reactions use a biphasic aqueous/organic solvent system that allows the catalyst to be reused. Reaction scales range from miligrams to grams, with 0.0086 to 0.12 mol% of antibody binding sites. Because antibodies 38C2 and 84G3 have opposite enantioselectivities, both aldol product enantiomers are accessible by kinetic resolution.
本文描述了使用醛缩酶抗体38C2（Aldrich编号47995-0）和84G3（Aldrich编号52785-8）对消旋醛醇1-4进行制备规模的动力学拆分。这些反应使用双相水/有机溶剂体系，使得催化剂能够重复使用。反应规模从毫克到克，抗体结合位点的用量为0.0086至0.12 mol%。由于抗体38C2和84G3具有相反的对映选择性，通过动力学拆分可以得到两种醛醇产物对映体。
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