Vorísek J, Pokorný V
Arch Microbiol. 1975 Mar 10;102(3):293-8. doi: 10.1007/BF00428380.
Cytochemical staining in toto (periodic acid, thiosemicarbazide, OSO4) revealed the presence of polysaccharide lamellae on the surface of the cell membrane complex of fungi. The membraneous clusters in the vacuolar bodies of Claviceps purpurea were covered with these lamellae at both surfaces, as it was also the case with the endoplasmic reticulum membranes, the tonoplast and the cytoplasmic membrane. In Saccharomyces cerevisiae, the polysaccharide lamellae were visible on the surface of the endoplasmic reticulum membranes and the plasmalemma; the strain revealed polysaccharide deposits also on the tonoplasts of small vacuoles and in glucanase vesicles. We assume that these observations give precision to the localization of the enzymes synthetizing the glycoprotein components of the fungal cell wall.
整体细胞化学染色（高碘酸、硫代氨基脲、四氧化锇）显示真菌细胞膜复合体表面存在多糖片层。麦角菌液泡体中的膜性簇在其两面均被这些片层覆盖，内质网膜、液泡膜和细胞质膜也是如此。在酿酒酵母中，多糖片层在内质网膜和质膜表面可见；该菌株在小液泡的液泡膜和葡聚糖酶小泡中也显示有多糖沉积。我们认为这些观察结果明确了合成真菌细胞壁糖蛋白成分的酶的定位。
