普通变形杆菌产生的软骨素酶对硫酸软骨素和透明质酸的降解动力学。
The kinetic of degradation of chondroitin of sulphates and hyaluronic acid by chondroitinase form Proteus vulgaris.
作者信息
Thurston C F
出版信息
Biochem J. 1975 Feb;145(2):397-400. doi: 10.1042/bj1450397.
Abstract
Km and Vmax. were determined for the degradation by chondroitinase of chondroitin 4-sulphate, 4-sulphate-proteoglycna, chondroitin 6-sulphate, dermatan sulphate and hyaluronic acid. Degradation of chondroitin 4-sulphate was inhibited by hyaluronic acid but not by keratan sulphate. The results are discussed with regard to the use to the use of chondroitinase as a sleective reagent for the degradation of tissue glycosaminoglycans.
摘要
测定了软骨素酶对硫酸软骨素4、4-硫酸化蛋白聚糖、硫酸软骨素6、硫酸皮肤素和透明质酸的降解作用的米氏常数（Km）和最大反应速度（Vmax）。透明质酸可抑制硫酸软骨素4的降解，但硫酸角质素无此作用。结合软骨素酶作为组织糖胺聚糖降解的选择性试剂的用途对这些结果进行了讨论。
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