通过高效液相色谱法测定人尿中透明质酸、硫酸软骨素和硫酸皮肤素的不饱和二糖。

Determination of human urinary hyaluronic acid, chondroitin sulphate and dermatan sulphate as their unsaturated disaccharides by high-performance liquid chromatography.

作者信息

Toyoda H, Motoki K, Tanikawa M, Shinomiya K, Akiyama H, Imanari T

机构信息

Faculty of Pharmaceutical Sciences, Chiba University, Japan.

出版信息

J Chromatogr. 1991 Apr 19;565(1-2):141-8. doi: 10.1016/0378-4347(91)80378-p.

DOI:10.1016/0378-4347(91)80378-p

PMID:1908476

Abstract

A method for the determination of hyaluronic acid (HA), chondroitin sulphate (CS) and dermatan sulphate (DS) was developed. HA, CS and DS were converted to the corresponding unsaturated disaccharides by digestion with chondroitinase ABC and/or chondroitinase AC-II and determined by high-performance liquid chromatography with fluorimetric detection using 2-cyanoacetamide as a post-column derivatization reagent. The calibration graphs for the unsaturated disaccharides were linear over the range 2 ng - 2 micrograms for each unsaturated disaccharide. This method was applied to the analysis of normal human urine.

摘要

开发了一种测定透明质酸（HA）、硫酸软骨素（CS）和硫酸皮肤素（DS）的方法。通过用软骨素酶ABC和/或软骨素酶AC-II消化，将HA、CS和DS转化为相应的不饱和二糖，并采用高效液相色谱法，以2-氰基乙酰胺作为柱后衍生试剂进行荧光检测来测定。每种不饱和二糖的不饱和二糖校准曲线在2 ng - 2 μg范围内呈线性。该方法应用于正常人尿液的分析。