Padilla L, Matus V, Zenteno P, González B
Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Alameda, Santiago, Chile.
J Basic Microbiol. 2000;40(4):243-9. doi: 10.1002/1521-4028(200008)40:4<243::AID-JOBM243>3.0.CO;2-D.
The aim of this work was to study the catabolic pathway of the pollutant 2,4,6-trichlorophenol in Ralstonia eutropha JMP134. 2,6-dichlorohydroquinone was detected as transient intermediate. Enzymatic transformations of 6-chlorohydroxyquinol to 2-chloromaleylacetate, and of this compound to maleylacetate were detected in crude extracts. Therefore, the degradation of 2,4,6-trichlorophenol proceeded through an hydroxyquinol pathway, different from the other chloroaromatic pathways reported in this strain. The same results were observed in two other 2,4,6-trichlorophenol degrading strains: R. eutropha JMP222, a derivative of strain JMP134 lacking the chlorocatechol catabolism-encoding pJP4 plasmid, and a river isolate, Ralstonia sp. PZK.
这项工作的目的是研究真养产碱菌JMP134中污染物2,4,6-三氯苯酚的分解代谢途径。检测到2,6-二氯对苯二酚为瞬时中间体。在粗提物中检测到6-氯羟基喹啉向2-氯马来酰乙酸以及该化合物向马来酰乙酸的酶促转化。因此，2,4,6-三氯苯酚的降解通过羟基喹啉途径进行，这与该菌株中报道的其他氯代芳烃途径不同。在另外两个降解2,4,6-三氯苯酚的菌株中也观察到了相同的结果：JMP222，它是JMP134菌株的衍生物，缺少编码氯儿茶酚分解代谢的pJP4质粒；以及一株从河流中分离出的菌株，拉尔斯通氏菌PZK。
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