Salman H, Bergman M, Weizman A, Bessler H, Weiss J, Straussberg R, Djaldetti M
Department of Medicine C, Rabin Medical Center, Petah Tiqva, Israel.
Biomed Pharmacother. 2000 Jul;54(6):311-5. doi: 10.1016/S0753-3322(00)80053-0.
To examine the effect of the anti-stress agent diazepam on the immune response, rats were exposed either to acute swim stress, i.e., swimming once only for 30 minutes (non-trained swimmers), or to chronic stress with gradual progressive training for 6 weeks (trained swimmers). Both groups of animals were divided into subgroups treated before swimming with and without diazepam, 1 mg/kg, administered i.m. The phagocytic capacity and superoxide anion generation of the peritoneal macrophages was examined. In addition, the proliferative response of the splenic cells (splenocytes) to concanavalin A (Con A) and phytohemagglutinin (PHA), as well as the serum corticosterone levels were investigated. Diazepam abolished increased superoxide anion production in both non-trained and trained swimmers. In non-trained swimmers, the number of latex particles internalized by each cell was decreased and further reduced by the drug. In trained rats treated with diazepam, the percentage of phagocytosing cells remained reduced compared to controls, whereas the drug prevented a decrease in the engulfing capacity of individual cells. Diazepam lowered the suppressed proliferative response of the splenocytes to PHA found in non-trained swimmers. It is concluded that diazepam modifies the immune response of rats exposed to acute and chronic swim stress.
为研究抗应激药物地西泮对免疫反应的影响，将大鼠分为两组，一组接受急性游泳应激，即仅游泳一次，持续30分钟（未训练的游泳大鼠），另一组接受慢性应激，即进行为期6周的渐进性训练（训练过的游泳大鼠）。两组动物又各自分为在游泳前接受或未接受1mg/kg地西泮腹腔注射的亚组。检测了腹腔巨噬细胞的吞噬能力和超氧阴离子生成情况。此外，还研究了脾细胞对刀豆蛋白A（Con A）和植物血凝素（PHA）的增殖反应以及血清皮质酮水平。地西泮消除了未训练和训练过的游泳大鼠中超氧阴离子生成的增加。在未训练的游泳大鼠中，每个细胞内化的乳胶颗粒数量减少，且药物使其进一步减少。在用了地西泮的训练大鼠中，与对照组相比，吞噬细胞的百分比仍降低，而药物阻止了单个细胞吞噬能力的下降。地西泮降低了未训练的游泳大鼠中脾细胞对PHA的增殖反应抑制。得出的结论是，地西泮改变了暴露于急性和慢性游泳应激的大鼠的免疫反应。
