Classen B, Witthohn K, Blaschek W
Department of Pharmaceutical Biology, Institute of Pharmacy, University of Kiel, Germany.
Carbohydr Res. 2000 Aug 7;327(4):497-504. doi: 10.1016/s0008-6215(00)00074-4.
An arabinogalactan-protein (AGP) from pressed juice of Echinacea purpurea herb was isolated from a high molecular weight fraction by precipitation with the beta-glucosyl Yariv reagent, followed by gel-permeation chromatography. It revealed characteristic features of other AGPs: i.e., a high amount of polysaccharide (83%) with a ratio of galactose to arabinose of 1.8:1, some uronic acids (4-5%), and a low protein content (7%) with high levels of serine, alanine and hydroxyproline. The molecular weight was estimated to be 1.2 x 10(6) Da. Linkage and 13C NMR analyses showed that the AGP is composed of a highly branched core polysaccharide of 3-, 6-, and 3,6-linked Galp residues with terminal Araf, GlcAp and terminal units of Araf-(1-->5)-Araf-(1-->. Partial acid hydrolysis resulted in loss of Araf residues at the periphery of the molecule. Complete loss of reactivity toward the beta-glucosyl Yariv antigen was then noticed.
紫锥菊草本压榨汁中的一种阿拉伯半乳聚糖蛋白（AGP），通过用β-葡萄糖基Yariv试剂沉淀，从高分子量组分中分离出来，随后进行凝胶渗透色谱分析。它展现出其他AGP的特征：即大量的多糖（83%），半乳糖与阿拉伯糖的比例为1.8:1，一些糖醛酸（4 - 5%），以及低蛋白含量（7%），其中丝氨酸、丙氨酸和羟脯氨酸含量较高。分子量估计为1.2×10⁶Da。连接和¹³C NMR分析表明，该AGP由高度分支的核心多糖组成，其含有3-、6-和3,6-连接的Galp残基，带有末端Araf、GlcAp以及Araf-(1→5)-Araf-(1→的末端单元。部分酸水解导致分子外围的Araf残基丢失。随后注意到对β-葡萄糖基Yariv抗原的反应性完全丧失。
