Classen Birgit, Blaschek Wolfgang
Pharmazeutisches Institut der Christian-Albrechts-Universität Kiel, Abteilung Pharmazeutische Biologie, Kiel, Germany.
Planta Med. 2002 Mar;68(3):232-6. doi: 10.1055/s-2002-23127.
An arabinogalactan-protein (AGP) from suspension culture medium of Malva sylvestris was isolated by precipitation with beta-glucosyl Yariv reagent, followed by gel-permeation chromatography. It revealed characteristic features of AGPs: a high amount of polysaccharide with a ratio of galactose to arabinose of 1.9 : 1, some uronic acids, and a small protein moiety with the main amino acids serine, alanine and hydroxyproline. The molecular weight was estimated to be 1.3 x 10(6) Da. Linkage analyses showed that the AGP is composed of a highly branched core polysaccharide of 3-, 6-, and 3,6-linked Galp residues with terminal Araf, GlcAp and Galp. Partial acid hydrolysis resulted in loss of Araf residues at the periphery of the molecule and heavily reduced its reactivity with beta-glucosyl Yariv antigen.
通过用β-葡萄糖基Yariv试剂沉淀，随后进行凝胶渗透色谱法，从锦葵悬浮培养基中分离出一种阿拉伯半乳聚糖蛋白（AGP）。它显示出AGP的特征：大量多糖，半乳糖与阿拉伯糖的比例为1.9 : 1，一些糖醛酸，以及一个小的蛋白质部分，主要氨基酸为丝氨酸、丙氨酸和羟脯氨酸。分子量估计为1.3×10⁶ Da。连接分析表明，该AGP由高度分支的核心多糖组成，该多糖由3-、6-和3,6-连接的Galp残基以及末端的Araf、GlcAp和Galp组成。部分酸水解导致分子外围的Araf残基丢失，并大大降低了其与β-葡萄糖基Yariv抗原的反应性。
