Gabridge M G, Yip D M, Hedges K
Infect Immun. 1975 Aug;12(2):233-9. doi: 10.1128/iai.12.2.233-239.1975.
The effects of a toxic dose of Mycoplasma fermentans on levels of lysosomal enzymes in mice were examined. Washed cell suspensions (approximately 10(10) colony-forming units) of a recent isolate of M. fermentans were injected intraperitoneally into 3- to 4-week-old BALB mice, and levels of acid phosphatase and beta-glucuronidase were monitored in liver, spleen, thymus, and serum. Levels of acid phosphatase remained essentially normal, but levels of beta-glucuronidase were markedly evevated in serum and to a lesser extent in liver and thymus. The peak response of serum beta-glucuronidase was noted at 8 h postinjection, with a level of 30 mug of phenolphthalein released per ml per h, representing a six-fold increase over control levels. Pretreatment with BCG did not potentiate the effect as it did with endotoxin. The implication of this increased lysosomal enzyme activity in "lethal toxicity" is that that the increase may be secondary to some other cytotoxic event, or that the affinity of mycoplasmas for biological membranes may be involved. The data suggest that the role of lysosomal enzymes in other models of mycoplasma-induced disease should be evaluated.
研究了毒性剂量的发酵支原体对小鼠溶酶体酶水平的影响。将近期分离的发酵支原体洗涤后的细胞悬液（约10¹⁰集落形成单位）腹腔注射到3至4周龄的BALB小鼠体内，并监测肝脏、脾脏、胸腺和血清中酸性磷酸酶和β-葡萄糖醛酸酶的水平。酸性磷酸酶水平基本保持正常，但血清中β-葡萄糖醛酸酶水平显著升高，肝脏和胸腺中的升高程度较小。注射后8小时观察到血清β-葡萄糖醛酸酶的峰值反应，每毫升每小时释放30微克酚酞，比对照水平增加了六倍。用卡介苗预处理并没有像对内毒素那样增强这种作用。这种溶酶体酶活性增加在“致死毒性”中的意义在于，这种增加可能是某些其他细胞毒性事件的继发结果，或者可能涉及支原体对生物膜的亲和力。数据表明，应评估溶酶体酶在支原体诱导疾病的其他模型中的作用。
