异亮氨酸四氮唑对大肠杆菌异亮氨酸生物合成的抑制作用。

Inhibition of Escherichia coli isoleucine biosynthesis by isoleucine tetrazole.

作者信息

Willshaw G A, Tristram H

出版信息

J Bacteriol. 1975 Sep;123(3):862-70. doi: 10.1128/jb.123.3.862-870.1975.

DOI:10.1128/jb.123.3.862-870.1975

PMID:1099080

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC235808/

Abstract

Growth of a derivative of Escherichia coli K-10 was strongly inhibited by 2 times 10(-4) M L-5(1-amino-2-methylbutyl)-tetrazole (isoleucine tetrazole). Growth inhibition was reversed by isoleucine, threonine, glycyl-L-isoleucine, or glycyl-L-threonine, and, in a valine-resistant mutant, by L-valine. Partial reversal of growth inhibiton was effected by L-leucine, L-methionine, or L-homoserine. The tetrazole inhibited the activity of the biosynthetic threonine deaminase (EC 4.2.1.16 L-threonine hydrolyase [deaminating]), the inhibition being relieved by L-valine. The tetrazole also inhibited isoleucyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.5 L-isoleucine: tRNA ligase [adenosine monophosphate]), but was without effect on the activities of alpha-isopropylmalate synthetase or acetohydroxy acid synthetase. One class of isoleucine tetrazole-resistant mutants produced biosynthetic threonine deaminases which were no longer subject to feedback inhibition by either isoleucine or the tetrazole.

摘要

大肠杆菌K - 10的一种衍生物的生长受到2×10⁻⁴M L - 5（1 - 氨基 - 2 - 甲基丁基） - 四唑（异亮氨酸四唑）的强烈抑制。异亮氨酸、苏氨酸、甘氨酰 - L - 异亮氨酸或甘氨酰 - L - 苏氨酸可逆转生长抑制，在缬氨酸抗性突变体中，L - 缬氨酸也可逆转生长抑制。L - 亮氨酸、L - 甲硫氨酸或L - 高丝氨酸可部分逆转生长抑制。四唑抑制生物合成苏氨酸脱氨酶（EC 4.2.1.16 L - 苏氨酸水解酶[脱氨]）的活性，L - 缬氨酸可解除这种抑制。四唑还抑制异亮氨酰 - 转移核糖核酸（tRNA）合成酶（EC 6.1.1.5 L - 异亮氨酸：tRNA连接酶[腺苷一磷酸]），但对α - 异丙基苹果酸合成酶或乙酰羟酸合成酶的活性没有影响。一类异亮氨酸四唑抗性突变体产生的生物合成苏氨酸脱氨酶不再受到异亮氨酸或四唑的反馈抑制。