异亮氨酸异羟肟酸介导的粘质沙雷氏菌生长抑制增强及异亮氨酸生产菌株的改良
Enhancement of isoleucine hydroxamate-mediated growth inhibition and improvement of isoleucine-producing strains of Serratia marcescens.
作者信息
Kisumi M, Komatsubara S, Chibata I
出版信息
Appl Environ Microbiol. 1977 Dec;34(6):647-53. doi: 10.1128/aem.34.6.647-653.1977.
Abstract
Growth inhibition by isoleucine hydroxamate in Serratia marcescens was significantly enhanced by adding valine plus leucine and by using glycerol as the carbon source. Isoleucine hydroxamate-resistant mutants were isolated under conditions in which growth inhibition was enhanced. One of the mutants, strain GIHVLr2179, lacked both feedback inhibition and repression of threonine deaminase. An alpha-aminobutyric acid-resistant mutant derived from strain GIHVLr2179, strain GIHVLAr2795, produced 12 mg of isoleucine per ml in the medium containing glucose and urea as carbon and nitrogen sources (a twofold increase over prior reports). This strain had increased activities of threonine deaminase, acetohydroxy acid synthase, aspartokinase, and homoserine dehydrogenase.
摘要
在粘质沙雷氏菌中，通过添加缬氨酸和亮氨酸以及使用甘油作为碳源，异亮氨酸氧肟酸盐对其生长的抑制作用显著增强。在生长抑制增强的条件下分离出了异亮氨酸氧肟酸盐抗性突变体。其中一个突变体GIHVLr2179菌株，缺乏苏氨酸脱氨酶的反馈抑制和阻遏。从GIHVLr2179菌株衍生出的α-氨基丁酸抗性突变体GIHVLAr2795菌株，在以葡萄糖和尿素作为碳源和氮源的培养基中，每毫升产生12毫克异亮氨酸（比之前的报道增加了两倍）。该菌株的苏氨酸脱氨酶、乙酰羟酸合酶、天冬氨酸激酶和高丝氨酸脱氢酶的活性有所增加。
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