Machaca K, Haun S
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA.
J Biol Chem. 2000 Dec 8;275(49):38710-5. doi: 10.1074/jbc.M007887200.
Store-operated calcium entry (SOCE) is the predominant Ca(2+) influx pathway in non-excitable cells and is activated in response to depletion of intracellular Ca(2+) stores. We have studied SOCE regulation during Xenopus oocyte meiosis. SOCE can be measured readily in stage VI Xenopus oocytes arrested at the G(2)-M transition of the cell cycle, either by Ca(2+) imaging or by recording the SOCE current. However, following meiotic maturation, SOCE can no longer be activated by store depletion. We have characterized the time course of SOCE inactivation during oocyte maturation, and show that SOCE inactivates almost completely, in a very short time period, at the germinal vesicle breakdown stage of meiosis. This acute inactivation offers an opportunity to better understand SOCE regulation.
储存性钙内流（SOCE）是非兴奋性细胞中主要的Ca(2+)内流途径，它在细胞内Ca(2+)储存耗尽时被激活。我们研究了非洲爪蟾卵母细胞减数分裂过程中的SOCE调节。通过Ca(2+)成像或记录SOCE电流，可以很容易地在处于细胞周期G(2)-M转换期停滞的VI期非洲爪蟾卵母细胞中测量SOCE。然而，减数分裂成熟后，SOCE不再能被储存耗尽激活。我们已经描述了卵母细胞成熟过程中SOCE失活的时间进程，并表明在减数分裂的生发泡破裂阶段，SOCE在很短的时间内几乎完全失活。这种急性失活为更好地理解SOCE调节提供了一个机会。
