Kääpä E, Wang W, Takala T E, Poussa M, Konttinen Y T, Lammi M J, Vanharanta H
Department of Physical Medicine and Rehabilitation, Helsinki University Hospital, Finland.
Connect Tissue Res. 2000;41(2):93-9. doi: 10.3109/03008200009067661.
Alterations involved with the intervertebral disc degeneration are partly well described, however, it is not so well known how collagen network is affected by the disease. We analyzed the rate of collagen biosynthesis (estimated by the enzymic activities of prolyl 4-hydroxylase and galactosylhydroxylysyl glucosyltransferase) and the level of hydroxylysylpyridinoline and lysylpyridinoline crosslinks both in normal (n=7) and degenerated (n=7) human annulus fibrosus. The activity of prolyl 4-hydroxylase was significantly increased in degenerated tissue. However, no significant changes in the collagen content or in the amount of hydroxylysylpyridinoline and lysylpyridinoline collagen crosslinks were observed. On the other hand, the content of soluble proteins was significantly increased. Our results suggest that collagen biosynthesis is increased in degenerated human annulus fibrosus, obviously to compensate the impairment of collagen fibers. The faster turnover of collagen in degenerated annulus fibrosus, suggested by the increased prolyl 4-hydroxylase activity and unchanged collagen content, seems not to cause any significant changes in its mature pyridinium crosslink concentrations.
虽然部分有关椎间盘退变的改变已有充分描述，但胶原蛋白网络如何受该疾病影响却鲜为人知。我们分析了正常（n = 7）和退变（n = 7）的人纤维环中胶原蛋白的生物合成速率（通过脯氨酰4-羟化酶和半乳糖基羟赖氨酰葡糖基转移酶的酶活性估算）以及羟赖氨酰吡啶啉和赖氨酰吡啶啉交联物的水平。在退变组织中，脯氨酰4-羟化酶的活性显著增加。然而，未观察到胶原蛋白含量或羟赖氨酰吡啶啉和赖氨酰吡啶啉胶原蛋白交联物的量有显著变化。另一方面，可溶性蛋白质的含量显著增加。我们的结果表明，退变的人纤维环中胶原蛋白生物合成增加，显然是为了补偿胶原纤维的损伤。脯氨酰4-羟化酶活性增加而胶原蛋白含量不变，这表明退变纤维环中胶原蛋白周转加快，但似乎并未使其成熟吡啶交联浓度发生任何显著变化。
