Department of Anatomy, University of Kuopio, Kuopio, Finland.
Eur Spine J. 1993 Oct;2(3):126-31. doi: 10.1007/BF00301408.
The effect of long-term excercise on the intervertebral disc collagen concentration (hydroxyproline), collagen-synthesizing enzymes (prolyl-4-hydroxylase, PH, and galactosyl-hydroxylysyl glucosyltransferase, GGT) and hydroxypyridinium crosslinks was studied in ten female beagle dogs. The dogs were run on a treadmill for 1 year starting at the age of 15 weeks. The daily running distance was gradually increased to 40km, which distance the dogs ran for the final 15 weeks. Ten untrained dogs from the same breeding colony served as controls. The nucleus pulposus and anterior and posterior halves of the annulus fibrosus of C2-3, T10-12, L4-5 disc segments were analysed. Crosslinks were measured from the anterior annulus fibrosus of the T10-11 disc. Hydroxyproline and hydroxypyridinium concentrations remained similar in both groups. PH and GGT were significantly elevated by running in the posterior annulus fibrosus of the thoracic and lumbar discs and in the lumbar nucleus pulposus. In the thoracic nucleus pulposus GGT was reduced significantly. The results suggest activated collagen metabolism in the posterior annulus fibrosus of the thoracic and lumbar discs as a result of locally increased strains on the spine.
本研究旨在探讨长期运动对椎间盘胶原浓度（羟脯氨酸）、胶原合成酶（脯氨酰-4-羟化酶、PH 和半乳糖基羟赖氨酸葡糖基转移酶、GGT）和羟吡啶交联物的影响。选用 10 只雌性比格犬，于 15 周龄时开始在跑步机上运动，持续 1 年。每日跑步距离逐渐增加至 40km，最后 15 周维持此距离。10 只来自同一繁殖群的未经训练的狗作为对照组。分析 C2-3、T10-12、L4-5 椎间盘节段的 C2-3 椎间盘的髓核和前、后纤维环的前、后半部分。从前纤维环的 T10-11 椎间盘测量交联物。两组的羟脯氨酸和羟吡啶浓度相似。运动使胸腰椎后纤维环和腰椎髓核中的 PH 和 GGT 显著升高。在胸髓核中，GGT 显著降低。结果表明，由于脊柱局部应变增加，胸腰椎后纤维环的胶原代谢活跃。
